Polycomb Responds to Low Levels of Transcription.
Polycomb Responds to Low Levels of Transcription.
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DOI：
10.1016/j.celrep.2017.06.076
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发表时间：
2017-07-25
期刊：
影响因子：
8.8
通讯作者：
Ptashne M
中科院分区：
文献类型：
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作者：
Berrozpe G;Bryant GO;Warpinski K;Spagna D;Narayan S;Shah S;Ptashne M
How is Polycomb (Pc), a eukaryotic negative regulator of transcription, targeted to specific mammalian genes? Our genome-wide analysis of the Pc mark H3K27me3 in murine cells that revealed Pc is preferentially associated with CpG island promoters of genes that are transcribed at a low level, and less so with promoters of genes that are either silent or more highly expressed. Studies of the CpG island promoter of the Kit gene demonstrate that Pc is largely absent when the gene is silent in myeloid cells as well as when the gene is highly expressed in mast cells. Manipulations that increase transcription in the former case, and reduce it in the latter, increase Pc occupancy. The average negative effect of Pc, we infer, is about two-fold. We suggest possible biological roles for such negative effects and propose a mechanism by which Pc might be recruited to weakly transcribed genes. Berrozpe et al. assayed occupancy of the polycomb mark H3K27me3 genome-wide and at a specific gene (Kit) when transcribed at different levels. They report that Polycomb is preferentially recruited to genes expressed at a low level.
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