Dendritic cells enhance growth and differentiation of CD40-activated B lymphocytes.
Dendritic cells enhance growth and differentiation of CD40-activated B lymphocytes.
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DOI：
10.1084/jem.185.5.941
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发表时间：
1997-03-03
期刊：
影响因子：
--
通讯作者：
Caux C
中科院分区：
文献类型：
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作者：
Dubois B;Vanbervliet B;Fayette J;Massacrier C;Van Kooten C;Brière F;Banchereau J;Caux C
捕获抗原后，树突状细胞 (DC) 迁移到次级淋巴器官富含 T 细胞的区域，在那里诱导 T 细胞激活，随后驱动 B 细胞激活。在这里，我们使用 CD40L 转染的 L 细胞作为替代激活的 T 细胞，研究体外产生的 DC 是否可以直接调节 B 细胞反应。 DC 通过产生可溶性介质，刺激 B 细胞增殖 3 至 6 倍并随后恢复。此外，在 CD40 连接后，DC 使 sIgD− B 细胞（本质上是记忆 B 细胞）分泌的 IgG 和 IgA 增强了 30-300 倍。在 DC 存在的情况下，初始 sIgD+ B 细胞响应白细胞介素 2 产生大量 IgM。因此，除了激活次级淋巴器官滤泡外区域的初始 T 细胞外，DC 还可以直接调节 B 细胞的生长和分化。
After antigen capture, dendritic cells (DC) migrate into T cell–rich areas of secondary lymphoid organs, where they induce T cell activation, that subsequently drives B cell activation. Here, we investigate whether DC, generated in vitro, can directly modulate B cell responses, using CD40L-transfected L cells as surrogate activated T cells. DC, through the production of soluble mediators, stimulated by 3- to 6-fold the proliferation and subsequent recovery of B cells. Furthermore, after CD40 ligation, DC enhanced by 30–300-fold the secretion of IgG and IgA by sIgD− B cells (essentially memory B cells). In the presence of DC, naive sIgD+ B cells produced, in response to interleukin-2, large amounts of IgM. Thus, in addition to activating naive T cells in the extrafollicular areas of secondary lymphoid organs, DC may directly modulate B cell growth and differentiation.