Effect of detergents and acid buffer on the reactivity of Fasciola hepatica antigens.
Effect of detergents and acid buffer on the reactivity of Fasciola hepatica antigens.
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去污剂和酸性缓冲液对肝片形吸虫抗原反应性的影响。
DOI：
10.2307/3280585
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
J. L. Rodríguez Ramos
中科院分区：
文献类型：
--
作者：
G. Hillyer;J. L. Rodríguez Ramos
研究了甘氨酸-盐酸缓冲液对抗原提取的影响以及三种常用去污剂-十二烷基硫酸钠（SDS）、脱氧胆酸钠（DOC）和Triton X-100-对标准肝片吸虫成虫抗原制剂（FhWWE）反应性的影响。用抗F抗体检测时，酸性缓冲液破坏了FhWWE的大部分免疫反应性。但显然不影响抗原与抗S.曼索尼血清这些研究表明，当FhWWE是抗体亲和色谱中使用的抗原提取物时，应谨慎使用酸性缓冲液洗脱。Triton X-100和DOC都没有明显增加FhWWE抗原的反应性。此外，DOC降低了抗原-抗体相互作用。低浓度的SDS增加了FhWWE的反应性，但较高浓度的SDS降低了抗原-抗体相互作用。
The effect of glycine-HCl (acid) buffer on antigen extraction and of three commonly used detergents--sodium dodecyl sulphate (SDS), sodium deoxycholate (DOC), and Triton X-100--on the reactivity of a standard Fasciola hepatica adult-worm antigen preparation (FhWWE) was studied. Acid buffer destroyed most of the immunologic reactivity of FhWWE when tested with anti-F. hepatica serum, but apparently did not affect the antigens reactivity with an anti-S. mansoni serum. These studies suggest that elution with acid buffer should be used with caution when FhWWE is the antigenic extract used in antibody-affinity chromatography. Neither Triton X-100 nor DOC appreciably increased the reactivity of FhWWE antigens. In addition, DOC decreased the antigen-antibody interactions. Low concentrations of SDS increased the reactivity of FhWWE, but higher concentrations decreased the antigen-antibody interactions.