Location of an extrinsic label in the primary and tertiary structure of bacteriorhodopsin.
Location of an extrinsic label in the primary and tertiary structure of bacteriorhodopsin.
复制标题
细菌视紫红质一级和三级结构中外在标记的位置。
DOI：
10.1016/s0006-3495(84)84013-8
复制
发表时间：
1984
影响因子：
3.4
通讯作者：
Hayward,SB
中科院分区：
文献类型：
--
作者：
Katre,NV;Finer-Moore,J;Stroud,RM;Hayward,SB
我们在细菌视紫红质的一级序列和三级结构中都定位了一个重金属标记，汞化的苯乙醛。该标记修饰了COOH-末端螺旋(G)上的精氨酸225和227。在投影的电子势差图中，主要位置靠近中心内螺旋。根据这一结果，我们认为螺旋1不可能是COOH末端的螺旋G。我们用电子衍射法测试了多重同晶置换法获得紫膜物相的方法。
We located a heavy metal label, mercurilated phenylglyoxal, in both the primary sequence and in the tertiary structure of bacteriorhodopsin. This label modified arginines 225 and 227, which are on the COOH-terminal helix (G). In the projected electron potential difference map, the major site is close to the central inner helix. From this result we conclude that helix 1 could not be the COOH-terminal helix G. We tested the multiple isomorphous replacement method for obtaining phases for purple membrane by electron diffraction.