Unsaturated glycerophospholipids mediate heme crystallization: biological implications for hemozoin formation in the kissing bug Rhodnius prolixus.
Unsaturated glycerophospholipids mediate heme crystallization: biological implications for hemozoin formation in the kissing bug Rhodnius prolixus.
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DOI：
10.1371/journal.pone.0088976
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Oliveira MF
中科院分区：
文献类型：
--
作者：
Stiebler R;Majerowicz D;Knudsen J;Gondim KC;Wright DW;Egan TJ;Oliveira MF
血球蛋白(HZ)是一些食血生物产生的一种血红素晶体，作为一种有效的脱毒方法，它是从血红蛋白消化中产生的。在三甲虫Rhodnius prolixus中，赫兹基本上是由中肠细胞外磷脂膜产生的，称为微绒毛周围膜(PMVM)。在这里，我们研究了以丝氨酸、胆碱和乙醇胺为头基的商品甘油磷脂和长春花杆菌中肠脂(RML)在血红素结晶中的作用。所有商业不饱和形式的磷脂以及RML都通过两种不同的动力学机制快速有效地介导β-hematin的形成：一种是早期和快速的成分，另一种是晚和慢的成分。不饱和形式的磷脂酰乙醇胺(UPE)和磷脂酰胆碱(UPC)诱导的反应最快，半衰期分别为0.04分钟和0.7分钟。β-血红素晶体形态在不同组之间有显著差异，UPE产生均匀规则的砖状晶体。有趣的是，UPC介导的反应导致了两个不同的晶体群：一个不太具代表性的规则晶体群，类似于UPE诱导的那些，另一个主要由具有大量尖锐边缘和锥形末端的晶体代表。RML诱导的血红素结晶反应是有效的，血红素向β-Hb的转化率高于70%，但明显慢于UPC和UPE的T1/2(9.9-17.7min)。有趣的是，RML产生的晶体形状均匀，与UPE介导的晶体非常相似。因此，不饱和甘油磷脂，特别是UPE和UPC能快速、有效地诱导β-Hb的形成，并可能在中肠血红素的生物结晶中发挥作用。
Hemozoin (Hz) is a heme crystal produced by some blood-feeding organisms, as an efficient way to detoxify heme derived from hemoglobin digestion. In the triatomine insect Rhodnius prolixus, Hz is essentially produced by midgut extracellular phospholipid membranes known as perimicrovillar membranes (PMVM). Here, we investigated the role of commercial glycerophospholipids containing serine, choline and ethanolamine as headgroups and R. prolixus midgut lipids (RML) in heme crystallization. All commercial unsaturated forms of phospholipids, as well as RML, mediated fast and efficient β-hematin formation by means of two kinetically distinct mechanisms: an early and fast component, followed by a late and slow one. The fastest reactions observed were induced by unsaturated forms of phosphatidylethanolamine (uPE) and phosphatidylcholine (uPC), with half-lives of 0.04 and 0.7 minutes, respectively. β-hematin crystal morphologies were strikingly distinct among groups, with uPE producing homogeneous regular brick-shaped crystals. Interestingly, uPC-mediated reactions resulted in two morphologically distinct crystal populations: one less representative group of regular crystals, resembling those induced by uPE, and the other largely represented by crystals with numerous sharp edges and tapered ends. Heme crystallization reactions induced by RML were efficient, with a heme to β-hematin conversion rate higher than 70%, but clearly slower (t1/2 of 9.9–17.7 minutes) than those induced by uPC and uPE. Interestingly, crystals produced by RML were homogeneous in shape and quite similar to those mediated by uPE. Thus, β-hematin formation can be rapidly and efficiently induced by unsaturated glycerophospholipids, particularly uPE and uPC, and may play a role on biological heme crystallization in R. prolixus midgut.
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影响因子：
4.3
作者：
Bewley, Kathryn D.;Ellis, Katie E.;Firer-Sherwood, Mackenzie A.;Elliott, Sean J.
通讯作者：
Elliott, Sean J.
DOI：
10.1039/b914359a
发表时间：
2010-02-07
期刊：
Dalton transactions (Cambridge, England : 2003)
影响因子：
--
作者：
Hoang AN;Ncokazi KK;de Villiers KA;Wright DW;Egan TJ
通讯作者：
Egan TJ
影响因子：
15.9
作者：
CHOU, AC;FITCH, CD
通讯作者：
FITCH, CD
影响因子：
3.8
作者：
de Villiers, Katherine A.;Osipova, Maria;Leiserowitz, Leslie
通讯作者：
Leiserowitz, Leslie
影响因子：
3.5
作者：
Egan, Timothy J.;Chen, Jeff Y-J.;Wood, Bayden R.
通讯作者：
Wood, Bayden R.