The inflammatory cell influx and cytokines changes during transition from acute inflammation to fibrous repair around implanted materials
The inflammatory cell influx and cytokines changes during transition from acute inflammation to fibrous repair around implanted materials
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DOI：
10.1163/156856206777346340
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发表时间：
2006-01-01
影响因子：
3.6
通讯作者：
Thomsen, Peter
中科院分区：
文献类型：
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作者：
Gretzer, Christina;Emanuelsson, Lena;Thomsen, Peter
使用表面化学性质不同但表面形貌相似的钛和组织培养聚苯乙烯(PS)圆盘，在可降解的聚氨基甲酸酯尿素(PUUR；Artelon((R)周围的皮下模型中评估了炎症和纤维反应。分别于植入后1、7、21d观察细胞因子、细胞活性、细胞反应、细胞分化及纤维包膜形成和血管形成情况。从眼袋中取出的渗出液进行细胞总数、细胞类型比例、单核/巨噬细胞分化(ED1、ED2)、DNA含量和存活率(LD、台盼蓝)的分析。用双抗体夹心法测定肿瘤坏死因子α(H)TNF-α和白细胞介素10(H)IL-10的含量。分析血管数目、管腔面积、血管分布及纤维包膜厚度。在愈合早期(1-7天)，所有种植体周围渗出液中的细胞数量最多。渗出液中ED2阳性细胞的比例由第1天的2~8%增加到第21天的43~56%。肿瘤坏死因子-α水平较低，7d时下降。21天后，渗出液中检测到大量IL-10，尤其是在PUUR附近。本研究表明，在PUUR、Ti和PS材料周围，从炎症到修复的转变(1-21天)的特征是炎性细胞内流减少，表达ED2的巨噬细胞比例增加，双相分泌TNF-α，IL-10增加，并形成与所有被测材料相似的纤维性包膜。
The inflammatory and fibrous responses in a subcutaneous rat model were evaluated around degradable polyurethane urea (PUUR; Artelon((R))), with titanium and tissue culture polystyrene (PS) discs having different surface chemical properties but similar surface topography. Cytokines, viability, cellular response, differentiation of cells and fibrous capsule formation and vascularization was investigated after 1, 7 and 21 days of implantation. The exudates retrieved from the pockets were analysed with respect to the total cell numbers, the proportions of cell types, the differentiation of monocytes/macrophages (ED1, ED2), the DNA content and the viability (LD, Trypan blue). Tumour necrosis factor alpha ((h)TNF-alpha) and interleukin-10 ((h)IL-10) were quantified by ELISA. The number of blood vessels, blood vessel luminal area, blood vessel distribution and the fibrous capsule thickness were analysed. The highest number of cells in the exudates around all implants was detected during the early phase of healing (1-7 days). The proportion of ED2-positive cells in the exudates increased from 2-8% at 1 day to 43-56% at 21 days. The levels of TNF-alpha were low with a decrease at 7 days. After 21 days high amounts of IL-10 in the exudates were detected, in particular around PUUR. This study shows that the transition from inflammation to repair (1-21 days) around PUUR, Ti and PS materials was characterized by a decrease in inflammatory cell influx, an increasing proportion of ED2-expressing macrophages, a biphasic TNF-alpha secretion, an increase of IL-10 and a fibrous capsule formation similar to all materials tested.