Silver nanoparticles alter bacterial assembly and antibiotic resistome in biofilm during colonization
Silver nanoparticles alter bacterial assembly and antibiotic resistome in biofilm during colonization
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银纳米粒子在定植过程中改变生物膜中的细菌组装和抗生素抗性组
DOI：
10.1039/d2en01018f
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Fang Tao
中科院分区：
文献类型：
--
作者：
Bao Shaopan;Xue Lu;Xiang Dongfang;Xian Bo;Tang Wei;Fang Tao
银纳米颗粒（AgNPs）越来越多地被排放到淡水系统中，在那里它们可能会遇到生物膜，并潜在地影响生物膜的形成和结构。然而，关于AgNPs在生物膜形成过程中对微生物群落组装和抗生素抗性组的影响知之甚少。在这项研究中，我们研究了AgNP暴露对微生物组装和结构的影响，以及抗生素耐药基因（ARG）的生物膜中的微生物定植成熟。在生物膜形成过程中，特别是在微生物定植的早期阶段，环境和临床相关水平的银纳米颗粒显着降低了微生物群落的生物量和多样性。此外，银纳米粒子有可能大大加快微生物群的演替速度，并在生物膜形成过程中改变微生物群落结构。模型分析表明，AgNP处理倾向于增加生物膜微生物群落形成过程中的确定性组装过程的比例。此外，暴露于临床相关水平（1 mg L−1）的AgNPs可能会轻微改变ARG的总体特征，并显着增加生物膜中总ARG的丰度。Procrustes分析和结构方程模型揭示了生物膜微生物群和ARG之间的显着关联。综上所述，这些结果表明，AgNPs进入淡水系统可能对生物膜微生物群和抗生素耐药性产生深远影响，突出了纳米颗粒的潜在生态风险。
Silver nanoparticles (AgNPs) are increasingly being discharged into freshwater systems, where they might encounter biofilm, and potentially affect the biofilm formation and structure. However, little is known about the effects of AgNPs on the microbial community assembly and antibiotic resistome during biofilm formation. In this study, we investigated the impacts of AgNP exposure on the microbial assembly and structure, and antibiotic resistance gene (ARG) profiles in the biofilm from microbial colonization to maturity. Both environmentally and clinically relevant levels of AgNPs significantly reduced the microbial community biomass and diversity during biofilm formation, particularly in the early stages of microbial colonization. In addition, AgNPs have the potential to profoundly accelerate the succession rate of microbiota and alter the microbial community structure during biofilm formation. Null model analyses indicated that the AgNP treatment tends to increase the ratio of the deterministic assembly process during the formation of biofilm microbial communities. Furthermore, exposure to AgNPs at a clinically relevant level (1 mg L−1) could marginally change the overall profiles of ARGs and significantly increase the abundance of total ARGs in the biofilm. Procrustes analysis and structural equation models revealed a significant association between biofilm microbiota and ARGs. Taken together, these results indicate that AgNPs entering freshwater systems might have profound impacts on biofilm microbiota and antibiotic resistome, highlighting the potential ecological risks of nanoparticles.