Single and double infections with Wolbachia in the parasitic wasp Nasonia vitripennis: effects on compatibility.
Single and double infections with Wolbachia in the parasitic wasp Nasonia vitripennis: effects on compatibility.
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DOI：
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发表时间：
1996-06
期刊：
影响因子：
3.3
通讯作者：
M. Perrot-Minnot;L. Guo;J. Werren
中科院分区：
文献类型：
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作者：
M. Perrot-Minnot;L. Guo;J. Werren
Wolbachia是一种细胞质遗传的细菌，在许多昆虫中引起生殖不相容性。然而，很少有人探索物种内的沃尔巴克氏体多态性及其对相容性的影响。在这里，我们表明，一些菌株的寄生蜂Nasonia vitripennis感染两种不同的细菌菌株（A和B），而其他人是单独感染（A或B）。通过PCR扩增和基因组DNA的Southern分析证实双重和单一感染。此外，它表明，延长幼虫滞育（越冬阶段的黄蜂）的双重感染菌株可能会导致随机损失的一个或两个细菌菌株。在双感染系滞育后，产生具有AB、仅A、仅B或不具有沃尔巴克氏体的亚系。A和B亚系是双向不亲和的，而AB系的雄性与A和B亚系的雌性是单向不亲和的。因此，结果表明，由于相关共生细菌的分离，物种内的双向不亲和性迅速发展。
Wolbachia are cytoplasmically inherited bacteria responsible for reproductive incompatibility in a wide range of insects. There has been little exploration, however, of within species Wolbachia polymorphisms and their effects on compatibility. Here we show that some strains of the parasitic wasp Nasonia vitripennis are infected with two distinct bacterial strains (A and B) whereas others are singly infected (A or B). Double and single infections are confirmed by both PCR amplification and Southern analysis of genomic DNA. Furthermore, it is shown that prolonged larval diapause (the overwintering stage of the wasp) of a double-infected strain can lead to stochastic loss of one or both bacterial strains. After diapause of a double-infected line, sublines were produced with AB, A only, B only or no Wolbachia. A and B sublines are bidirectionally incompatible, whereas males from AB lines are unidirectionally incompatible with females of A and B sublines. Results therefore show rapid development of bidirectional incompatibility within a species due to segregation of associated symbiotic bacteria.