Effects of brief perturbations in energy balance on indices of glucose homeostasis in healthy lean men
Effects of brief perturbations in energy balance on indices of glucose homeostasis in healthy lean men
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DOI：
10.1038/ijo.2011.211
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发表时间：
2012-08-01
影响因子：
4.9
通讯作者：
Mueller, M. J.
中科院分区：
文献类型：
--
作者：
Lagerpusch, M.;Bosy-Westphal, A.;Mueller, M. J.
背景技术背景：关于短期热量限制（CR）和过度喂养（OF）对健康瘦个体葡萄糖稳态的影响知之甚少。此外，目前还不清楚CR和OF的影响是否逆转的补充feeding period.METHODS：10名健康男性参加了两个周期的控制7天期间的CR和refeeding（RF;协议A），和OF和CR（协议B）在+/- 60%的能量需求。在基线时，通过正葡萄糖钳夹（M）评估胰岛素敏感性（IS）。在每个喂养周期之前和期间，使用空腹和口服葡萄糖耐量试验衍生的指数来估计葡萄糖耐量、IS和葡萄糖刺激的胰岛素分泌。结果：钳夹试验显示基线IS正常（M值9.4 +/- 2.1 mg kg（-1）min（-1），变异系数（CV）（inter）22%）。M值与IS指数显著相关。在方案A中，CR诱导的体重减轻（-3.0 +/-0.4 kg）与空腹IS增加相关。餐后IS和葡萄糖刺激的胰岛素分泌保持不变，但葡萄糖耐量降低。RF降低空腹和餐后IS增加葡萄糖刺激的胰岛素分泌。在方案B中，OF显著增加了体重（+1.6 +/-0.9 kg）。同时，空腹和餐后IS在葡萄糖刺激的胰岛素分泌增加时降低。随后的CR逆转了这些效应。结论：在控制性饥饿和低饲条件下，大鼠糖代谢指标的个体间变异性较大，变异系数在9%~ 59%之间。虽然IS在CR-RF周期结束时受损，但IS在OF-CR周期后恢复正常。在不同的喂养方案，葡萄糖代谢的稳态反应是高度可变的。
BACKGROUND: Little is known about the effects of short-term caloric restriction (CR) and overfeeding (OF) on glucose homeostasis in healthy lean individuals. In addition, it remains unclear whether the effects of CR and OF are reversed by a complementary feeding period.METHODS: Ten healthy men participated in two cycles of controlled 7-day periods of CR and refeeding (RF; protocol A), and OF and CR (protocol B) at +/- 60% energy requirement. At baseline, insulin sensitivity (IS) was assessed by euglycemic clamp (M). Before and during each feeding cycle, fasting and oral glucose tolerance test-derived indices were used to estimate glucose tolerance, IS and glucose-stimulated insulin secretion.RESULTS: Clamp tests revealed normal IS at baseline (M-values 9.4 +/- 2.1 mg kg(-1) min(-1), coefficient of variation (CV)(inter) 22%). M-values were significantly correlated with indices of IS. In protocol A, CR-induced weight loss (-3.0 +/- 0.4 kg) was associated with an increase in fasting IS. Postprandial IS and glucose-stimulated insulin secretion remained unchanged, but glucose tolerance decreased. RF decreased fasting and postprandial IS at increased glucose-stimulated insulin secretion. In protocol B, OF significantly increased the body weight (+1.6 +/- 0.9 kg). Concomitantly, fasting and postprandial IS decreased at increased glucose-stimulated insulin secretion. Subsequent CR reversed these effects. Inter-individual variability in indices of glucose metabolism was high with coefficients of variation ranging from 9 to 59%.CONCLUSION: Significant changes in glucose metabolism are evident within 7-day periods of controlled OF and underfeeding. Although IS was impaired at the end of the CR-RF cycle, IS was normalized after the OF-CR cycle. At different feeding regimens, homeostatic responses of glucose metabolism were highly variable.