Adult-onset calorie restriction attenuates kainic acid excitotoxicity in the rat hippocampal slice
Adult-onset calorie restriction attenuates kainic acid excitotoxicity in the rat hippocampal slice
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DOI：
10.1016/j.neulet.2007.11.064
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发表时间：
2008-01-31
影响因子：
2.5
通讯作者：
McRae, Amanda
中科院分区：
文献类型：
--
作者：
Youssef, Farid F.;Ramchandani, Jaya;McRae, Amanda
终身卡路里限制是唯一已知的干预措施，已被证明可以持续延长寿命并减少衰老对大脑的影响。考虑到在人类群体中复制终身卡路里限制的困难，我们试图评估成人短期卡路里限制对大鼠海马区急性兴奋性毒性侮辱的影响。成年动物(大约6个月大)接受7-10周的卡路里限制(隔日喂养)。利用电生理和免疫细胞化学技术，我们报告了卡路里限制对长时程增强(LTP)没有影响，LTP是神经功能的一种衡量标准。在对照动物中，使用红藻氨酸(20亩M)仅能使伤后CA1群体峰电位恢复35%。然而，限制卡路里摄入的动物在红藻氨酸处理后表现出明显的恢复(%)。这一数据得到了免疫细胞化学研究的支持，该研究指出，在海人酸处理后，对照切片中微管相关蛋白(MAP 2)免疫反应性广泛丧失；然而，在卡路里限制动物的CA1和CA3区，MAP 2染色得到保留。有趣的是，当N-甲基-D-天冬氨酸(15mM)处理切片时，不同组之间的群体穗恢复没有显着差异。我们的结论是，短期成人开始的卡路里限制不会改变正常的神经元功能，并有助于保护海马体免受急性红藻氨酸兴奋毒性的影响。(C)2007爱思唯尔爱尔兰有限公司。保留所有权利。
Lifelong calorie restriction is the only known intervention that has been shown to consistently increase life span and reduce the effects of aging on the brain. Given the difficulties of replicating lifelong calorie restriction within human populations, we have sought to assess the effects of short-term adult-onset calorie restriction upon acute excitotoxic insults in the rat hippocampus. Adult animals (approximately 6 months of age) underwent calorie restriction (alternate day feeding) for 7-10 weeks. Utilizing both electrophysiological and immunocytochemical techniques, we report that calorie restriction had no effect upon long-term potentiation (LTP), a measure of neuronal function. In control animals, application of kainic acid (20 mu M) resulted in only 35% recovery of CA1 population spikes post-insult. However calorie-restricted animals showed significantly improved recovery after kainic acid treatment (64%). This data was supported by immunocytochemical studies which noted widespread loss of microtubule-associated protein (MAP 2) immunoreactivity in control slices following treatment with kainic acid; however MAP 2 staining was preserved in the CA1 and CA3 regions of calorie-restricted animals. Interestingly there was no significant difference in the recovery of population spikes between groups when slices were treated with N-methyl-D-aspartate (15 mu M). We conclude that short-term adult-onset calorie restriction does not alter normal neuronal function and serves to protect the hippocampus from acute kainic acid excitotoxicity. (c) 2007 Elsevier Ireland Ltd. All rights reserved.