CD26/dipeptidyl-peptidase IV in psoriatic skin: upregulation and topographical changes
CD26/dipeptidyl-peptidase IV in psoriatic skin: upregulation and topographical changes
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DOI：
10.1111/j.1365-2133.2008.08515.x
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发表时间：
2008-06-01
影响因子：
10.3
通讯作者：
van Erp, P. E. J.
中科院分区：
文献类型：
--
作者：
van Lingen, R. G.;de Kerkhof, P. C. M. van;van Erp, P. E. J.
背景银屑病影响2-3%的人群，可被认为是一种器官特异性自身免疫性疾病。CD26/二肽基肽酶IV(DPP-IV)是一种具有多种性质的膜结合蛋白水解酶。目的探讨CD26/DPP-IV在银屑病中的表达，为寻找与银屑病炎症和增殖相关的互补生物标志物奠定基础。方法采用免疫组织化学、免疫荧光和酶活性标记技术，从mRNA、蛋白质和酶功能水平研究CD26/DPP-IV在银屑病发病机制中的表达。银屑病切片免疫组织化学显示，CD26/DPP-IV在乳头上层有明显的斑片状蜂窝状染色。此外，在沿着网脊的整个基底上室可见明显可区分的柱状染色图案，而在正常皮肤中这些图案是不存在的。值得注意的是，CD26/DPP-IV酶活性与CD26/DPP-IV蛋白的这种免疫组织化学反应模式相关。CD26/DPP-IV在银屑病患者皮肤中的T细胞结合表达明显存在，尽管数量很少。结论我们的研究结果为CD26/DPP-IV在银屑病真皮中的表达普遍上调提供了明确的证据。尽管确切的功能贡献仍然是推测的，这种复杂的多功能蛋白的地形分布表明作为银屑病的补充生物标记物是合适的。
Background Psoriasis is known to affect 2-3% of the population and can be considered an organ-specific autoimmune disease. CD26/dipeptidyl-peptidase IV (DPP-IV) is a membrane-bound protease with diverse properties. In theory, the expression of CD26/DPP-IV has common grounds with three principal key players of the psoriatic pathogenesis: keratinocytes, T cells and cytokines.Objectives To assess CD26/DPP-IV expression in psoriasis in order to expand on the search for complementary biomarkers related to inflammation and proliferation in psoriasis.Methods The pattern of expression of CD26/DPP-IV was investigated on the mRNA-, protein- and enzyme-functionality level using immunohistochemical, immunofluorescent and enzyme activity labelling techniques.Results An 11-fold significant increase of CD26/DPP-IV on the mRNA level was demonstrated in psoriatic epidermal sheets compared with normal skin. Immunohistochemistry on psoriatic sections showed a distinct patchy honeycomb-like CD26/DPP-IV staining in the suprapapillary layers. Moreover, a clearly distinguishable column-like staining pattern throughout the suprabasal compartment along the rete ridges was seen, whereas in normal skin these patterns were absent. Strikingly, CD26/DPP-IV enzyme activity correlated with this immunohistochemical reactivity pattern for the CD26/DPP-IV protein. The T-cell bound expression of CD26/DPP-IV in psoriatic skin was explicitly present, albeit in small quantities.Conclusions Our data provide clear evidence for a versatile upregulation of CD26/DPP-IV expression in psoriatic (epi)dermis. Although the exact functional contribution remains speculative, the topographical distribution of this complex multifunctional protein suggests a suitable role as a complementary biomarker in psoriasis.