PRC2-mediated H3K27me3 modulates shoot iron homeostasis in Arabidopsis thaliana
PRC2-mediated H3K27me3 modulates shoot iron homeostasis in Arabidopsis thaliana
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DOI：
10.1080/15592324.2020.1784549
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发表时间：
2020-06
影响因子：
2.9
通讯作者：
Emily Y Park;Kaitlyn M. Tsuyuki;Elizabeth M. Parsons;Jeeyon Jeong
中科院分区：
文献类型：
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作者：
Emily Y Park;Kaitlyn M. Tsuyuki;Elizabeth M. Parsons;Jeeyon Jeong
摘要 植物利用复杂的机制来适应不断变化的铁条件，因为铁是必需的，也是植物生长最限制的营养素之一。此外，铁过量可能有毒，必须严格监管。此前，我们发现，在缺铁条件下，通过组蛋白 3 赖氨酸 27 三甲基化 (H3K27me3) 进行的染色质重塑可调节 FIT 依赖性基因的表达。这项研究建立在我们之前的研究结果的基础上，表明 H3K27me3 也调节芽中的铁调节。在缺乏主要 H3K27 三甲基转移酶的 clf 突变体中，我们检测到与野生型相比，缺铁条件下向芽的铁易位增加。对芽的转录组分析还揭示了基因的差异表达，这与在缺铁条件下clf芽中铁含量高于野生型芽一致。此外，我们验证了 YSL1 和 IMA1（参与从芽到根的铁状态信号传导的两个基因）是 H3K27me3 的直接目标，并揭示了 H3K27me3 在这些位点上的铁依赖性沉积。这项研究有助于更好地了解植物铁调节背后的分子机制，因为据我们所知，PRC2 介导的 H3K27me3 对芽中表达的铁稳态基因的影响此前尚未有报道。
ABSTRACT Plants use intricate mechanisms to adapt to changing iron conditions because iron is essential and also one of the most limiting nutrients for plant growth. Furthermore, iron is potentially toxic in excess and must be tightly regulated. Previously, we showed that chromatin remodeling via histone 3 lysine 27 trimethylation (H3K27me3) modulates the expression of FIT-dependent genes under iron deficiency in roots. This study builds on our previous findings, showing that H3K27me3 also modulates iron regulation in shoots. In the clf mutant, which lacks the predominant H3K27 tri-methyltransferase, we detected increased iron translocation to shoots under iron deficiency as compared to wild type. Transcriptomic analysis of shoots also revealed differential expression of genes consistent with higher iron levels in clf shoots than wild type shoots under iron-deficient conditions. In addition, we verify that YSL1 and IMA1, two genes involved in signaling iron status from shoots to roots, are direct targets of H3K27me3 and reveal iron-dependent deposition of H3K27me3 on these loci. This study contributes to a better understanding of the molecular mechanisms behind iron regulation in plants, as the effect of PRC2-mediated H3K27me3 on iron homeostasis genes expressed in the shoots has not been previously reported to our knowledge.