Determination of Dopamine in the Presence of Ascorbic Acid using Poly (Acridine red) Modified Glassy Carbon Electrode
Determination of Dopamine in the Presence of Ascorbic Acid using Poly (Acridine red) Modified Glassy Carbon Electrode
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DOI：
10.3390/s31000443
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发表时间：
2003-10
期刊：
影响因子：
3.9
通讯作者：
Yuzhong Zhang;Guiying Jin;Yan-Ling Wang;Zhousheng Yang
中科院分区：
文献类型：
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作者：
Yuzhong Zhang;Guiying Jin;Yan-Ling Wang;Zhousheng Yang
采用聚吖啶红修饰玻碳电极，采用循环伏安法和差分脉冲伏安法检测pH为7.4的磷酸缓冲溶液（PBS）中抗坏血酸存在下的多巴胺。聚吖啶红膜对阳离子多巴胺和阴离子抗坏血酸具有明显的吸引能力，克服了抗坏血酸氧化电位重叠的主要困难。结果表明，多巴胺阳极峰电流与多巴胺浓度在1.0×10-7 ~ 1.0×10-4 mol dm-3范围内呈线性关系。差分脉冲伏安法检测限（S/N=3）为1.0×10-9 mol dm-3。1.0×10-6 mol dm-3 DA连续10次扫描的相对标准偏差为2.07%。抗坏血酸对多巴胺的测定几乎没有干扰。该方法具有良好的回收率和重现性。
Poly (acridine red) modified glassy carbon electrode was used for the detection of dopamine in the presence of ascorbic acid in a pH 7.4 phosphate buffer solutions (PBS) by cyclic voltammetry and differential pulse voltammetry. The major difficulty of the overlapped oxidation potential of ascorbic acid could be overcome through the distinct attractive ability of poly (acridine red) film to cationic dopamine and anionic ascorbic acid. The results showed that the dopamine anodic peak current and the concentration of dopamine had a linear relationship in the range of 1.0×10-7 ~ 1.0×10-4 mol dm-3. The detection limit (S/N=3) obtained by differential pulse voltammetry was 1.0×10-9 mol dm-3. The relative standard deviation of 10 successive scans was 2.07 % for 1.0×10-6 mol dm-3 DA. Ascorbic acid had hardly interference with the determination of dopamine. The proposed method exhibits good recovery and reproducibility.