Aptamer-conjugated gold functionalized graphene oxide nanocomposites for human α-thrombin specific recognition
Aptamer-conjugated gold functionalized graphene oxide nanocomposites for human α-thrombin specific recognition
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DOI：
10.1016/j.chroma.2015.12.018
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发表时间：
2016-01-04
影响因子：
4.1
通讯作者：
Zhang, Yukui
中科院分区：
文献类型：
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作者：
Deng, Nan;Jiang, Bo;Zhang, Yukui
对复杂生物样品中靶蛋白的特异性识别在临床诊断和治疗中具有巨大的潜力，受到越来越多的关注。在此，我们通过适配体共轭金功能化氧化石墨烯纳米复合材料（表示为Apt/Au/PEI/GO纳米复合材料）实现了人血清α -凝血酶的特异性检测。以聚亚胺为还原剂和稳定剂，在氧化石墨烯表面原位生长金纳米粒子，合成了金功能化氧化石墨烯纳米复合材料，并通过形成Au- s键作为载体固定化适配体。所得的Apt/Au/PEI/GO纳米复合材料不仅具有较大的表面积，使适配体的固定化量高达36.1 nmol/mg，而且具有优异的亲水性，即使在3000倍人血清蛋白的干扰下也能对人α -凝血酶特异性识别。此外，Apt/Au/PEI/GO纳米复合材料具有优异的捕获效率（约86%）和优异的识别重复性。最后，Apt/Au/PEI/GO纳米复合材料成功应用于人血清α -凝血酶特异性识别，验证了其在临床应用中的巨大潜力。(C) 2015 Elsevier B.V.版权所有
The specific recognition toward target proteins from complex biological samples has great potential in clinical diagnostics and therapeutics, receiving more and more attention. Herein, we achieved the specific detection of human alpha-thrombin from human serum by aptamer-conjugated gold functionalized graphene oxide nanocomposites (denoted as Apt/Au/PEI/GO nanocomposites). Gold functionalized graphene oxide nanocomposites were synthesized by in situ growth of Au nanoparticles on graphene oxide surface using polyethylenimine as reducing and stabilizing reagents, and then it was used as support for aptamer immobilization through forming an Au-S bonding. The obtained Apt/Au/PEI/GO nanocomposites inherited not only the large surface area which made the immobilizing amount of aptamer up to 36.1 nmol/mg, but also the excellent hydrophilicity which showed remarkable selectivity for human alpha-thrombin specific recognition, even with the interference of 3000 fold human serum proteins. Furthermore, with its superior properties, Apt/Au/PEI/GO nanocomposites showed advantages of high capture efficiency (>86%) and excellent recognition repeatability. Finally, the Apt/Au/PEI/GO nanocomposites were successfully applied for human alpha-thrombin specific recognition in human serum, verifying its great potential in clinical applications. (C) 2015 Elsevier B.V. All rights reserved.