Quartz crystal microbalance based biosensors for detecting highly metastatic breast cancer cells via their transferrin receptors
Quartz crystal microbalance based biosensors for detecting highly metastatic breast cancer cells via their transferrin receptors
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DOI：
10.1039/c5ay02898a
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发表时间：
2016-01-01
影响因子：
3.1
通讯作者：
Denizli, Adil
中科院分区：
文献类型：
--
作者：
Atay, Seda;Piskin, Kevser;Denizli, Adil
将转铁蛋白修饰在金传感器表面，构建了一种用于检测高转移性乳腺癌细胞的石英晶体微天平（QCM）生物传感器。使用具有高转移潜能和转铁蛋白表达的MDA-MB 231乳腺癌细胞和具有低转移潜能和转铁蛋白表达的MCF 7细胞。血清饥饿的MDA-MB-231细胞用作对照细胞。首先，以甲基丙烯酸羟乙酯（HEMA）和二甲基丙烯酸乙二醇酯（EGDMA）为原料，通过细乳液聚合制备了聚甲基丙烯酸羟乙酯（PHEMA）纳米粒子。纳米颗粒的特征在于与zeta-sizer，然后将其悬浮液滴在QCM的表面上，干燥的QCM表面进一步通过活化与碳二亚胺和转铁蛋白连接改性。采用原子力显微镜（AFM）、椭圆偏振光谱仪、傅里叶变换红外光谱仪（FTIR）和接触角测量等方法对QCM生物传感器进行了分析。将细胞应用于衍生化的QCM传感器以研究亲和性和结合动力学。发现纳米颗粒和转铁蛋白在QCM表面上形成单层。结合动力学最适合于Langmuir-Freundlich吸附模型。QCM信号与细胞上的转铁蛋白受体的数量相关性良好。结论：QCM生物传感器通过转铁蛋白受体的相互作用，可用于检测乳腺癌细胞的高转移潜能。
A quartz crystal microbalance (QCM) biosensor was developed to detect highly metastatic breast cancer cells by functionalizing the gold sensor surface with transferrin attachment. MDA-MB 231 breast cancer cells with high and MCF 7 cells with low metastatic potential and transferrin expression were used. Serum starved MDA-MB-231 cells were used as control cells. First, poly(2-hydroxyethyl methacrylate) (PHEMA) nanoparticles were prepared by mini-emulsion polymerization of hydroxyethyl methacrylate (HEMA) and ethylene glycol dimethacrylate (EGDMA). Nanoparticles were characterized with a zeta-sizer and then their suspension is dropped on the surface of the QCM and the dried QCM surface was modified further by activation with carbodiimide and transferrin attachment. The QCM biosensor was analyzed by using atomic force microscopy (AFM), ellipsometry, Fourier transform infrared spectrophotometry (FTIR) and contact angle measurements. The cells were applied to the derivatized QCM sensor to investigate the affinity and binding kinetics. The nanoparticles and transferrin were found to form a monolayer on the QCM surface. Binding kinetics were best fitted to the Langmuir-Freundlich adsorption model. The QCM signal was correlated well with the number of transferrin receptors on cells. It is concluded that the QCM biosensor functioning via transferrin receptor interactions may be used to detect breast cancer cells with high metastatic potential.