Galectin-3 expression is associated with bladder cancer progression and clinical outcome
Galectin-3 expression is associated with bladder cancer progression and clinical outcome
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DOI：
10.1007/s13277-010-0033-9
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发表时间：
2010-08-01
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通讯作者：
Sanchez-Carbayo, Marta
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作者：
Canesin, Giacomo;Gonzalez-Peramato, Pilar;Sanchez-Carbayo, Marta
半乳糖凝集素-3属于碳水化合物结合蛋白家族，其功能尚未完全表征。然而，它被认为在实体瘤中的粘附、增殖和凋亡中起作用。我们的目的是研究半乳糖凝集素-3在膀胱癌中的表达。半乳糖凝集素-3表达通过转录谱（U133 A阵列）在一系列或冷冻膀胱肿瘤（n = 105）中进行评估。对包含膀胱肿瘤的组织阵列（n = 389）进行免疫组织化学，以评估半乳糖凝集素-3蛋白表达模式与增殖（Ki 67）、凋亡（Adhesidetek）、bcl-2和临床病理变量的相关性。然后通过酶免疫分析定量160例膀胱癌患者和对照组尿液标本中的半乳糖凝集素-3蛋白水平。与非肌肉浸润性病变相比，浸润性肿瘤中半乳糖凝集素-3基因表达水平增加（p=0.001），并与晚期疾病患者的生存率低相关（p=0.03）。galectin-3蛋白表达模式与肿瘤分期相关（p
Galectin-3 belongs to a family of carbohydrate-binding proteins whose function is not fully characterized. However, it is believed to play a role in adhesion, proliferation and apoptosis in solid tumors. We aimed at investigating galectin-3 expression in bladder cancer. Galectin-3 expression was assessed by transcript profiling (U133A arrays) in a series or frozen bladder tumors (n = 105). Immunohistochemistry was performed on tissue arrays containing bladder tumors (n = 389) to evaluate associations of protein expression patterns of galectin-3 with proliferation (Ki67), apoptosis (apopdetek), bcl-2, and clinicopathologic variables. Galectin-3 protein levels were then quantified in 160 urinary specimens of bladder cancer patients and controls by enzymeimmunoanalysis. Galectin-3 gene expression levels increased in invasive tumours as compared with non-muscle invasive lesions (p=0.001) and were associated with poor survival in patients with advanced disease (p=0.03). Protein expression patterns also correlated galectin-3 with tumor stage (p