Optimisation of asymmetrical flow-field fractionation for the characterisation of gum arabic (Acacia sengal var senegal) and comparison with gel permeation chromatography
Optimisation of asymmetrical flow-field fractionation for the characterisation of gum arabic (Acacia sengal var senegal) and comparison with gel permeation chromatography
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DOI：
10.1039/c3ay40835c
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发表时间：
2013-01-01
影响因子：
3.1
通讯作者：
Jackson, Kevin
中科院分区：
文献类型：
--
作者：
Andres-Brull, Marina;Al-Assaf, Saphwan;Jackson, Kevin
采用与三种检测系统(多角度激光散射法、折射率法和紫外法)联用的不对称流动-流动分馏(AF4)技术，测定了塞内加尔相思胶(以下简称阿拉伯胶)的相对分子质量和分子分布。考察了样品浓度和外加电场强度(横流)的影响，以确定最佳的分级条件，并与单分散多糖(普鲁兰-100)的行为进行了比较。AF4-MALLS与GPC-MALLS的预测结果吻合较好。
Asymmetrical flow field-flow fractionation (AF4) linked to three detection systems (multi angle laser light scattering, refractive index and UV) was used to determine the molecular weight and molecular distribution of the component Acacia sengal var senegal (henceforth referred to as gum arabic). The effects of sample concentration and applied field strength (cross-flow) were examined to determine the optimum fractionation conditions in comparison with the behaviour of a monodispersed polysaccharide (Pullulan-100). The results using AF4-MALLS are in good agreement with those using GPC-MALLS.