Influence of bacteria and gentamicin on cooled-stored stallion spermatozoa
Influence of bacteria and gentamicin on cooled-stored stallion spermatozoa
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DOI：
10.1016/j.theriogenology.2006.11.004
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发表时间：
2007-03-15
期刊：
影响因子：
2.8
通讯作者：
Spergser, J.
中科院分区：
文献类型：
--
作者：
Aurich, C.;Spergser, J.
本研究调查了马生殖道细菌的影响以及精液稀释液中庆大霉素对马冷冻精液精子功能的影响。精液取自健康公马，用含或不含庆大霉素的乳基稀释液(1g/L)进行精液处理。假单胞菌(Passomonas(Ps.)铜绿假单胞菌、葡萄球菌(St.)金黄色葡萄球菌、链球菌(SC.)Equi亚种。Equi(SC.Equi)，SC.Equi亚种。兽疫线虫(Sc.ZooEpemicus)，SC.乳酸菌亚种。等同(SC.加入等价物)或单独加入培养基作对照。在添加细菌后即刻，在5℃保存24、48和72h后，用CASA系统测定稀释精液的活力、速度和膜完整性。24小时后，精液中加入P。铜绿假单胞菌和沙门氏菌。与所有其他组相比，等同组的运动速度和运动速度显著降低；72h后，Ps的运动和速度仍然存在这种差异。铜绿假单胞菌(p<；0.05)。SC孵育24 h后，精子膜完整性百分率显著降低。等效性，72h后加入SC。Equisimilis和P.铜绿假单胞菌。在无菌精液中添加庆大霉素后，精子活力和运动速度降低，添加细菌后精子运动参数无明显改善。总而言之，某些细菌在冷藏过程中可能会对精液质量产生不利影响。这些作用不能通过添加庆大霉素来减少。在冷冻保存过程中，庆大霉素会对延长精液中的精子功能产生负面影响，因此，必须测试不同浓度的延长精液的最佳浓度。(C)2006 Elsevier Inc.保留所有权利。
This study investigated effects of bacteria from the genital tract of horses and the effect of gentamicin in semen extender on spermatozoal function in cooled-stored stallion semen. Semen was collected from healthy stallions and processed with a milk-based extender with or without gentamicin (1 g/l). Pseudomonas (Ps.) aeruginosa, Staphylococcus (St.) aureus, Streptococcus (Sc.) equi subsp. equi (Sc. equi), Sc. equi subsp. zooepidemicus (Sc. zooepidemicus), Sc. dysgalactiae subsp. equisimilis (Sc. equisimilis) or culture medium alone (control) were added. Immediately after addition of bacteria and after storage at 5 degrees C for 24, 48 and 72 h, motility, velocity and membrane integrity of diluted semen were determined with a CASA system. After 24 h, semen with Ps. aeruginosa and Sc. equisimilis showed significantly lower motility and velocity compared to all other groups; after 72 h these differences still existed for Ps. aeruginosa (p < 0.05). The percentage of membrane-intact spermatozoa was significantly lower after 24 h of storage in spermatozoa incubated with Sc. equisimilis and after 72 h with Sc. equisimilis and Ps. aeruginosa. Addition of gentamicin to extender resulted in decreased motility and velocity in semen without addition of bacteria and did not improve motility parameters in semen with bacteria added. In conclusion, certain bacteria may have detrimental effects on semen quality during cooled-storage. These effects are not reduced by addition of gentamicin. Gentamicin can negatively affect spermatozoal function in extended semen during cooled-storage and therefore, optimal concentrations have to be tested for the respective extender medium. (c) 2006 Elsevier Inc. All rights reserved.