Combined effect of fertilizer and herbicide applications on the abundance, community structure and performance of the soil methanotrophic community
Combined effect of fertilizer and herbicide applications on the abundance, community structure and performance of the soil methanotrophic community
复制标题
DOI：
10.1016/j.soilbio.2004.05.025
复制
发表时间：
2005-02
影响因子：
9.7
通讯作者：
D. Seghers;S. Siciliano;E. Top;W. Verstraete
中科院分区：
文献类型：
--
作者：
D. Seghers;S. Siciliano;E. Top;W. Verstraete
在农业系统中使用农用化学品，如矿物肥料和除草剂，可能会影响土壤作为甲烷汇的潜力。通常情况下，每种农用化学品对土壤甲烷氧化的影响是单独研究的，而在实地，这些农用化学品一起使用，形成一个综合的土地管理系统。在这里，我们报告了田间试验的结果，评估了多种肥料和除草剂（烟嘧磺隆，二甲苯酰胺和莠去津）的应用对土壤甲烷氧化菌群落的综合影响。与接受矿物肥料的土壤相比，用有机肥料处理的土壤的甲烷氧化率高出三倍。这些更高的氧化速率积极反映在显着增强丰富的甲烷氧化菌的有机施肥土壤。与此相反，除草剂的应用并没有改变显着的土壤甲烷氧化速率或甲烷氧化人口丰度。随后，甲烷氧化菌群落结构进行了分析与组特异性DGGE的16S rRNA基因。甲烷营养模式的聚类分析清楚地将矿物与有机肥土壤分开。不太明显的聚类区分化学和手动杂草控制。聚类分析表明，土壤类型是影响甲烷氧化菌群落结构的主要因素。我们的研究结果表明，肥料类型对甲烷氧化剂活性和丰度的影响最大。土壤类型对微生物群落结构的影响最为显著。
The use of agrochemicals, such as mineral fertilizers and herbicides in agricultural systems, may affect the potential of soils to act as a sink for methane. Typically, the effect of each agrochemical on soil methane oxidation is investigated separately whereas in the field these agrochemicals are used together to form one comprehensive land management system. Here we report the results of field experiments that assessed the combined effect of multiple fertilizer and herbicide (nicosulfuron, dimethenamide and atrazine) applications on the soil methanotrophic community. Soils treated with organic fertilizer had three times higher methane oxidation rates compared to soils receiving mineral fertilizers. These higher oxidation rates were positively reflected in a significantly enhanced abundance of methanotrophs for the organic fertilized soils. In contrast, herbicide application did not alter significantly the soil methane oxidation rate or the methane-oxidizing population abundance. Subsequently, the methanotrophic community structure was analyzed with group-specific DGGE of 16S rRNA genes. Cluster analysis of the methanotrophic patterns clearly separated the mineral from organically fertilized soils. Less pronounced clustering differentiated between chemical and manual weed control. Furthermore, cluster analysis of the methanotrophic community revealed that soil type was the primary determinant of the community structure. Our results indicate that fertilizer type had the greatest influence on methane oxidizer activity and abundance. Soil type had the most pronounced effect on the microbial community structure.