The distribution of fitness effects of new beneficial mutations in Pseudomonas fluorescens
The distribution of fitness effects of new beneficial mutations in Pseudomonas fluorescens
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DOI：
10.1098/rsbl.2010.0547
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发表时间：
2011-02-23
期刊：
影响因子：
3.3
通讯作者：
Rainey, Paul B.
中科院分区：
文献类型：
--
作者：
McDonald, Michael J.;Cooper, Tim F.;Rainey, Paul B.
适应的理论研究强调了了解新突变的适应度效应(DFE)分布的重要性。我们报告了从一个祖先基因中分离出100个适应性突变体--没有自然选择的偏见影响--该基因在派生类型所占据的生态位中的适合度极低。每种衍生基因相对于单一参考类型的适合度被确定，并且适合度效应被发现符合正态分布。当在不同的环境中测量适合度时，排名顺序改变了，但分布的形状没有改变。我们认为，即使对支持适应性类型的遗传结构有详细的了解(就像这里的情况)，DFE仍然是不可预测的，我们讨论了一种可能性，即在缺乏生物体特有的生物学细节的情况下，DFE的形状可能不可能得到普遍的解释。
Theoretical studies of adaptation emphasize the importance of understanding the distribution of fitness effects (DFE) of new mutations. We report the isolation of 100 adaptive mutants-without the biasing influence of natural selection-from an ancestral genotype whose fitness in the niche occupied by the derived type is extremely low. The fitness of each derived genotype was determined relative to a single reference type and the fitness effects found to conform to a normal distribution. When fitness was measured in a different environment, the rank order changed, but not the shape of the distribution. We argue that, even with detailed knowledge of the genetic architecture underpinning the adaptive types (as is the case here), the DFEs remain unpredictable, and we discuss the possibility that general explanations for the shape of the DFE might not be possible in the absence of organism-specific biological details.