A-kinase anchoring protein 3 messenger RNA expression in ovarian cancer and its implication on prognosis
A-kinase anchoring protein 3 messenger RNA expression in ovarian cancer and its implication on prognosis
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DOI：
10.1002/ijc.11565
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发表时间：
2004-01-01
影响因子：
6.4
通讯作者：
Nakayama, E
中科院分区：
文献类型：
--
作者：
Hasegawa, K;Ono, T;Nakayama, E
A激酶锚定蛋白3（AKAP 3）是一种精子蛋白，其表达似乎仅限于正常成人组织中的睾丸。我们采用逆转录-聚合酶链反应（RT-PCR）检测了20例正常卵巢组织和54例不同组织学类型、分级和分期的卵巢癌组织中AKAP 3 mRNA的表达。PCR产物通过常规琼脂糖凝胶电泳和微尖装置上的毛细管电泳进行分析，以半定量地确定表达。在20例正常卵巢标本中几乎没有表达。AKAP 3 mRNA在15例卵巢癌组织中呈高表达（28%）.其表达与组织学分级及临床分期有关。AKAP 3 mRNA在低分化（p = 0.009）和晚期（III和IV，p = 0.014）肿瘤中以显著更高的频率观察到。AKAP 3 mRNA表达与其他变量之间无相关性。在考克斯多变量分析中，发现AKAP 3 mRNA表达是低分化肿瘤患者总体生存期和无进展生存期的重要预测因子。(C)2003 Wiley-Liss，Inc.
A-kinase anchoring protein 3 (AKAP3) is a sperm protein and its expression appears to be restricted to the testis in normal adult tissues. We investigated AKAP3 mRNA expression in 20 normal ovaries and 54 ovarian cancers of different histological types, grades and stages by reverse transcription-polymerase chain reaction (RT-PCR). The PCR products were analyzed by conventional agarose gel electrophoresis and capillary electrophoresis on a microtip device to determine the expression semiquantitatively. Little or no expression was observed in the 20 normal ovarian specimens. High AKAP3 mRNA expression was observed in 15 ovarian cancer specimens (28 %). The expression was correlated with the histological grade and clinical stage. AKAP3 mRNA was observed at a significantly higher frequency in poorly differentiated (p = 0.009) and advanced stage (I I I and IV, p = 0.014) tumors. No correlation was found between AKAP3 mRNA expression and other variables. In Cox multivariate analysis, AKAP3 mRNA expression was found to be a significant predictor of both overall and progression-free survival in patients with poorly differentiated tumors. (C) 2003 Wiley-Liss, Inc.