Biosynthesis of the pyoverdine siderophore of Pseudomonas aeruginosa involves precursors with a myristic or a myristoleic acid chain
Biosynthesis of the pyoverdine siderophore of Pseudomonas aeruginosa involves precursors with a myristic or a myristoleic acid chain
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DOI：
10.1016/j.febslet.2011.12.004
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发表时间：
2012-01-02
期刊：
影响因子：
3.5
通讯作者：
Schalk, Isabelle J.
中科院分区：
文献类型：
--
作者：
Hannauer, Melissa;Schaefer, Mathias;Schalk, Isabelle J.
吡咯烷酮I(PVDI)是铜绿假单胞菌进口铁的主要铁载体。这种螯合剂的生物合成涉及非核糖体多肽合成酶和其他酶。PVDQ是NTN水解酶家族中的一种周质酶，参与PVDI生物合成的最后几步。PvdQ突变体产生两个非荧光的PVDI前体，其分子质量高于PVDI。在本研究中，我们描述了利用质谱学来确定这些PVDI前体的结构，并表明它们都含有一个未形成的发色团，如铁蛋白，以及在PVDI分泌到细胞外介质之前必须去除的肉豆蔻酸链或肉豆蔻酸链。(C)2011年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Pyoverdine I (PVDI) is the major siderophore produced by Pseudomonas aeruginosa to import iron. Biosynthesis of this chelator involves non-ribosomal peptide synthetases and other enzymes. PvdQ is a periplasmic enzyme from the NTN hydrolase family and is involved in the final steps of PVDI biosynthesis. A pvdQ mutant produces two non-fluorescent PVDI precursors with a higher molecular mass than PVDI. In the present study, we describe the use of mass spectrometry to determine the structure of these PVDI precursors and show that they both contain a unformed chromophore like ferribactin, and either a myristic or myristoleic chain that must be removed before PVDI is secreted into the extracellular medium. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.