ENDOTHELIN SUBTYPE-B RECEPTORS ARE COUPLED TO ADENYLATE-CYCLASE VIA INHIBITORY-G PROTEIN IN CULTURED BOVINE ENDOTHELIAL-CELLS
ENDOTHELIN SUBTYPE-B RECEPTORS ARE COUPLED TO ADENYLATE-CYCLASE VIA INHIBITORY-G PROTEIN IN CULTURED BOVINE ENDOTHELIAL-CELLS
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DOI：
10.1097/00005344-199322008-00043
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发表时间：
1993-01-01
影响因子：
3
通讯作者：
MARUMO, F
中科院分区：
文献类型：
--
作者：
EGUCHI, S;HIRATA, Y;MARUMO, F
我们研究了内皮素 (ET) 异肽是否对培养的牛内皮细胞 (EC) 中的腺苷酸环化酶活性有影响。 ET-1和ET-3均剂量依赖性地抑制毛喉素和异丙肾上腺素刺激的cAMP形成，尽管ET-1的抑制作用不如ET-3有效。相比之下，在用磷酰胺（一种假定的 ET-1 转换酶抑制剂）预处理的牛 EC 中，ET-1 和 ET-3 几乎同等地抑制毛喉素刺激的 cAMP 形成。这些数据表明内皮 ET(B) 受体可能通过 G(i) 蛋白与腺苷酸环化酶功能性偶联。
We studied whether endothelin (ET) isopeptides have any effects on adenylate cyclase activity in cultured bovine endothelial cells (ECs). Both ET-1 and ET-3 dose-dependently inhibited cAMP formation stimulated by forskolin and isoproterenol, although the inhibitory effect of ET-1 was less potent than that of ET-3. In contrast, ET-1 and ET-3 almost equipotently inhibited forskolin-stimulated cAMP formation in bovine EC pre-treated with phosphoramidon, a putative ET-1-converting-enzyme inhibitor. These data suggest that endothelial ET(B) receptors are functionally coupled to adenylate cyclase, possibly via G(i) protein.