CHARACTERIZATION OF INCOMPATIBILITY GROUP HI1 PLASMIDS FROM SALMONELLA-TYPHI BY RESTRICTION ENDONUCLEASE DIGESTION AND HYBRIDIZATION OF DNA PROBES FOR TN3, TN9, AND TN10
CHARACTERIZATION OF INCOMPATIBILITY GROUP HI1 PLASMIDS FROM SALMONELLA-TYPHI BY RESTRICTION ENDONUCLEASE DIGESTION AND HYBRIDIZATION OF DNA PROBES FOR TN3, TN9, AND TN10
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DOI：
10.1139/m85-136
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发表时间：
1985-01-01
影响因子：
2.8
通讯作者：
BROSE, EC
中科院分区：
文献类型：
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作者：
TAYLOR, DE;BROSE, EC
伤寒沙门氏菌对氯霉素的耐药性是由不相容群H亚群1(IncHI1)的质粒介导的。用限制性内切酶对来自墨西哥、越南、泰国和印度的8株伤寒沙门氏菌的IncHI1质粒进行了检测。限制性内切酶ApaI、XbaI和PstI被发现对比较质粒DNA最有用。1972至1974年间，从墨西哥、越南和泰国分离的伤寒沙门氏菌的四个质粒对所有三种酶都有相同的限制性内切酶图谱。其他的IncHI1质粒仅表现出微小的差异。然而，这些IcHI1与1961年从鼠伤寒沙门氏菌中分离到的IncHI1原生粒R27有一些显著的差异，并已构建了其限制性内切酶图谱。以缺口翻译的HI1质粒为探针的Southern转移杂交证实，IncHI1质粒之间存在大量的序列同源性。用DNA探针定位氨苄西林耐药(Tn3)、氯霉素耐药(Tn9)、四环素耐药(Tn10)的DNA序列，以及IncHI1与F因子的单向不相容(IncHI1质粒的特性)。结果表明，从不同地理来源的伤寒沙门氏菌分离的IncHI1质粒非常相似。伤寒沙门氏菌与R27的比较表明，DNA的保守区是参与接合转移的区域。
Chloramphenicol resistance in Salmonella typhi is mediated by plasmids of the incompatibility group H, subgroup 1 (IncHI1). Eight IncHI1 plasmids from S. typhi strains originating in Mexico, Vietnam, Thailand, and India were examined by restriction enzyme digestion. The restriction enzymes, ApaI, XbaI, and PstI were found to be most useful for comparison of plasmid DNAs. Four plasmids from S. typhi isolated in Mexico, Vietnam, and Thailand between 1972 and 1974 had identical restriction patterns with all three enzymes. The other IncHI1 plasmids showed only minor differences. However, some significant differences were noted between these IcHI1 plasmids and the prototype IncHI1 plasmid R27, which was isolated from S. typhimurium in 1961 and for which a restriction map has been constructed. Southern transfer hybridization with a nick-translated HI1 plasmid as a probe confirmed that there is a great deal of sequence homology among the IncHI1 plasmids. DNA probes were used to locate DNA sequences for ampicillin resistance (Tn3), chloramphenicol resistance (Tn9), tetracycline resistance (Tn10), and the one-way incompatibility between IncHI1 plasmids and the F factor, a characteristic property of IncHI1 plasmids. The results demonstrate that IncHI1 plasmids isolated from S. typhi from widely different geographic sources are very similar. Comparisons between the S. typhi plasmids and R27 indicated that conserved regions of DNA were those involved in conjugative transfer.