A carbohydrate-grafted nanovesicle with activatable optical and acoustic contrasts for dual modality high performance tumor imaging.
A carbohydrate-grafted nanovesicle with activatable optical and acoustic contrasts for dual modality high performance tumor imaging.
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具有可激活光学和声学对比的碳水化合物接枝纳米囊泡，用于双模态高性能肿瘤成像
DOI：
10.1039/c4sc03641g
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发表时间：
2015-03-01
期刊：
影响因子：
8.4
通讯作者：
Han S
中科院分区：
文献类型：
--
作者：
Wu X;Lin B;Yu M;Yang L;Han J;Han S
唾液酸靶向酸响应型纳米微囊内化到肿瘤溶酶体后变成荧光和光声，从而实现了皮下肿瘤和肝脏肿瘤病灶的高性能照明。能够精确定位肿瘤的可激活分子系统对于完整的肿瘤切除是很有价值的。在此，我们报道了包裹近红外前荧光团(pNIR@P@SA)的唾液酸包裹的聚合物纳米微囊，用于基于溶酶体激活的双模式肿瘤成像。该探针的特点是表面锚定的唾液酸用于肿瘤靶向和近红外前荧光团(PNIR)的核心，它经历溶酶体酸性触发的异构化，在细胞内化时给出光学和光声信号。成像研究显示，pNIR@P@SA在小鼠皮下肿瘤和毫米级肝癌病灶中高效摄取和信号激活。肿瘤与健康器官信号的高对比度和对微小肝肿瘤与正常肝组织的区分验证了pNIR@P@SA在高性能光学和光声成像引导下进行肿瘤切除的潜力。
High-performance illumination of subcutaneous tumor and liver tumor foci was achieved with sialic acid-targeted acid-responsive nanovesicles which become fluorescent and photoacoustic upon internalization into tumor lysosomes. Activatable molecular systems enabling precise tumor localization are valuable for complete tumor resection. Herein, we report sialic acid-capped polymeric nanovesicles encapsulating the near infrared profluorophore (pNIR@P@SA) for lysosome activation based dual modality tumor imaging. The probe features surface-anchored sialic acid for tumor targeting and a core of near infrared profluorophore (pNIR) which undergoes lysosomal acidity triggered isomerization to give optical and optoacoustic signals upon cell internalization. Imaging studies reveal high-efficiency uptake and signal activation of pNIR@P@SA in subcutaneous tumors and millimeter-sized liver tumor foci in mice. The high tumor-to-healthy organ signal contrasts and discernment of tiny liver tumors from normal liver tissues validate the potential of pNIR@P@SA for high performance optical and optoacoustic imaging guided tumor resection.