Cloning of rat hepatic lipase cDNA: evidence for a lipase gene family.
Cloning of rat hepatic lipase cDNA: evidence for a lipase gene family.
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DOI：
10.1073/pnas.84.6.1526
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发表时间：
1987-03
影响因子：
11.1
通讯作者：
M. Komaromy;M. Schotz
中科院分区：
文献类型：
--
作者：
M. Komaromy;M. Schotz
大鼠肝脂肪酶的克隆通过用基于部分肽序列合成的寡核苷酸探测λ gt 11中的大鼠肝cDNA文库来分离。克隆的信使编码472个氨基酸的蛋白质加上22个氨基酸的疏水前导序列。未糖基化蛋白的预测分子量为53，222，含有两个潜在的N-糖基化位点。该蛋白具有与其他已知脂肪酶显著同源的区域，并含有与脂质结合有关的肽序列。同源mRNA存在于肝脏组织中，但在肾上腺中未观察到可检测的mRNA，尽管报告了肝脏和肾上腺中均存在肝脂肪酶。在各种其他组织中均未观察到mRNA。
Clones for rat hepatic lipase were isolated by probing a rat liver cDNA library in lambda gt11 with an oligonucleotide synthesized on the basis of a partial peptide sequence. The cloned messenger codes for a protein of 472 amino acids plus a hydrophobic leader sequence of 22 amino acids. The unglycosylated protein has a predicted molecular weight of 53,222 and contains two potential sites for N-glycosylation. The protein bears striking regions of homology with other known lipases and contains peptide sequences that have been implicated in lipid binding. The homologous mRNA is present in liver tissue but no detectable mRNA is observed in the adrenal gland, despite the reported presence of hepatic lipase in both the liver and the adrenal gland. No mRNA was seen in any of a variety of other tissues.