A redox-responsive regulator of photosynthesis gene expression in the cyanobacterium Synechocystis sp strain PCC 6803
A redox-responsive regulator of photosynthesis gene expression in the cyanobacterium Synechocystis sp strain PCC 6803
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DOI：
10.1128/jb.182.15.4268-4277.2000
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发表时间：
2000-08-01
影响因子：
3.2
通讯作者：
Sherman, LA
中科院分区：
文献类型：
--
作者：
Li, H;Sherman, LA
我们已经确定了单细胞蓝细菌集胞藻属菌株PCC 6803中的基因，这些基因涉及光合作用的氧化还原控制和色素相关基因。基因rppA（sll 0797）和rppB（sll 0798）代表了一个双组分调控系统，该系统控制光系统IE（PSII）和PSI基因的合成，以及色素相关基因的合成。rppA。（光合作用和色素相关基因表达的调节子）和rppB分别与原核反应调节子和组氨酸激酶具有很强的序列相似性。在野生型中，PSII反应中心基因的稳态mRNA水平增加时，质体醌（PQ）池被氧化，减少时，PQ池被减少，而PSI反应中心基因的转录受到影响，在相反的方式。这些结果表明，PQ池的氧化还原平衡对于光系统反应中心基因的调节是至关重要的。在三角洲rppA，插入突变的rppA，PSII基因的转录高度上调相对于野生型在所有氧化还原条件下，而藻胆体相关基因和PSI基因的转录减少。在Delta rppA中psbA基因的较高转录表现为D1蛋白的较高翻译和伴随的O-2进化的增加。结果表明，RppA是一个调节光合作用和色素相关的基因表达，参与建立适当的化学计量之间的光系统，并可以感知PQ氧化还原平衡的变化。
We have identified genes in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 that are involved,vith redox control of photosynthesis and pigment-related genes. The genes, rppA (sll0797) and rppB (sll0798), represent a two-component regulatory system that controls the synthesis of photosystem IE (PSII) and PSI genes, in addition to photopigment-related genes. rppA. (regulator of photosynthesis- and photopigment-related gene expression) and rppB exhibit strong sequence similarity to prokaryotic response regulators and histidine kinases, respectively. In the wild type, the steady-state mRNA levels of PSII reaction center genes increased when the plastoquinone (PQ) pool was oxidized and decreased when the PQ pool was reduced, whereas transcription of the PSI reaction center genes was affected in an opposite fashion. Such results suggested that the redox poise of the PQ pool is critical for regulation of the photosystem reaction center genes. In Delta rppA, an insertion mutation of rppA, the PSII gene transcripts were highly up-regulated relative to the wild type under all redox conditions, whereas transcription of phycobilisome-related genes and PSI genes was decreased. The higher transcription of the psbA gene in Delta rppA was manifest by higher translation of the D1 protein and a concomitant increase in O-2 evolution. The results demonstrated that RppA is a regulator of photosynthesis- and photopigment-related gene expression, is involved in the establishment of the appropriate stoichiometry between the photosystems, and can sense changes in the PQ redox poise.