N-terminal sequence in ovalbumin.

N-terminal sequence in ovalbumin.
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卵清蛋白中的 N 末端序列。

DOI:
10.1093/oxfordjournals.jbchem.a127629
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发表时间:
1962
影响因子:
2.7
通讯作者:
J. Ishii
J. Ishii
中科院分区:
生物学4区
文献类型:
--
作者:
K. Narita;J. Ishii

文献摘要

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P orter(1)的报告指出,通过S anger(2)的二硝基氟苯法在卵清蛋白中检测不到游离-α-氨基,这导致了大量的努力来表征卵清蛋白的N-末端结构。作者实验室的T itani* 使用Sanger法和Edman异硫氰酸苯酯法(3)仔细地重复了卵清蛋白的N-末端分析,同样没有发现存在未取代的N-末端氨基酸的证据。
The report by P orter (1) that no free-a-amino group is detectable in ovalbumin by the dinitrofluorobenzene method of S anger (2) has led to numerous efforts to characterize the N-terminal structure of ovalbumin. Using both S anger's method and E dman's phenylisothiocyanate method (3), T itani* of the author's laboratory has carefully repeated the N-terminal analysis of ovalbumin and has likewise found no evidence for the presence of an unsubstituted N-terminal amino acid.