Development of a Contractile Cardiac Fiber From Pluripotent Stem Cell Derived Cardiomyocytes.
Development of a Contractile Cardiac Fiber From Pluripotent Stem Cell Derived Cardiomyocytes.
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DOI：
10.3389/fcvm.2018.00052
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发表时间：
2018
影响因子：
3.6
通讯作者：
Gaudette GR
中科院分区：
文献类型：
--
作者：
Hansen KJ;Laflamme MA;Gaudette GR
干细胞疗法有可能在心肌梗死后再生心功能。在这项研究中，我们试图检验是否可以利用纤维蛋白微线技术来开发人多能干细胞来源的心肌细胞(HPS-CM)的收缩纤维。种植在纤维蛋白微线上的HPS-CM能够附着在微线上，并在初始种植后7天开始收缩。将数字散斑跟踪算法应用于高速视频数据(>；60Fps)，以确定收缩行为，包括拍频、平均和最大收缩应变，以及21天内HPS-CM在微线上收缩的主收缩角。接种于组织培养塑料上的细胞在第7天的搏动频率为0.83±0.25次/秒，平均收缩应变为4.23±0.23%，与第21天的搏动频率1.11±0.45次/秒和平均收缩应变3.08±0.19%有显著差异(n=18，p<；0.05)。种植在微丝上的HPS-CM以0.84±0.15次/秒的频率跳动，平均收缩应变为3.56±0.22%，第21天分别增加到1.03±0.19次/秒和4.47±0.29%(n=18，p<；0.05)。在第7天，27%的细胞的收缩角度在微线的20度以内，而在第21天，65%的HPS-CM收缩在微线的20度以内(n=17)。利用Fluo-4AM负载HPS-CM种子微线的高速钙瞬变数据(300fps)，传导速度从第7天的3.69±1.76 cm/S显著增加到第21天的24.26±8.42 cm/S(n=5-6，p<；0.05)。HPS-CM接种的微线显示细胞间缝隙连接蛋白43呈阳性表达。这些数据表明，纤维蛋白微线是一种适合HPS-CM附着和收缩的支架。此外，延伸培养允许细胞向着丝线方向收缩，这表明细胞在微线方向上对齐。
Stem cell therapy has the potential to regenerate cardiac function after myocardial infarction. In this study, we sought to examine if fibrin microthread technology could be leveraged to develop a contractile fiber from human pluripotent stem cell derived cardiomyocytes (hPS-CM). hPS-CM seeded onto fibrin microthreads were able to adhere to the microthread and began to contract seven days after initial seeding. A digital speckle tracking algorithm was applied to high speed video data (>60 fps) to determine contraction behaviour including beat frequency, average and maximum contractile strain, and the principal angle of contraction of hPS-CM contracting on the microthreads over 21 days. At day 7, cells seeded on tissue culture plastic beat at 0.83 ± 0.25 beats/sec with an average contractile strain of 4.23±0.23%, which was significantly different from a beat frequency of 1.11 ± 0.45 beats/sec and an average contractile strain of 3.08±0.19% at day 21 (n = 18, p < 0.05). hPS-CM seeded on microthreads beat at 0.84 ± 0.15 beats/sec with an average contractile strain of 3.56±0.22%, which significantly increased to 1.03 ± 0.19 beats/sec and 4.47±0.29%, respectively, at 21 days (n = 18, p < 0.05). At day 7, 27% of the cells had a principle angle of contraction within 20 degrees of the microthread, whereas at day 21, 65% of hPS-CM were contracting within 20 degrees of the microthread (n = 17). Utilizing high speed calcium transient data (>300 fps) of Fluo-4AM loaded hPS-CM seeded microthreads, conduction velocities significantly increased from 3.69 ± 1.76 cm/s at day 7 to 24.26 ± 8.42 cm/s at day 21 (n = 5–6, p < 0.05). hPS-CM seeded microthreads exhibited positive expression for connexin 43, a gap junction protein, between cells. These data suggest that the fibrin microthread is a suitable scaffold for hPS-CM attachment and contraction. In addition, extended culture allows cells to contract in the direction of the thread, suggesting alignment of the cells in the microthread direction.
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影响因子：
8.4
作者：
Dhillon，Paramdeep S.;Gray，Rosaire;Peters，Nicholas S.
通讯作者：
Peters，Nicholas S.
影响因子：
24
作者：
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作者：
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ARZBAECHER, RC
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作者：
Lundy, Scott D.;Zhu, Wei-Zhong;Laflamme, Michael A.
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3.7
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Christoforou N;Liau B;Chakraborty S;Chellapan M;Bursac N;Leong KW
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