Histone H3 methylation at lysine 4 on the SLC2A5 gene in intestinal Caco-2 cells is involved in SLC2A5 expression
Histone H3 methylation at lysine 4 on the SLC2A5 gene in intestinal Caco-2 cells is involved in SLC2A5 expression
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DOI：
10.1016/j.bbrc.2009.12.136
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发表时间：
2010-01-29
影响因子：
3.1
通讯作者：
Goda, Toshinao
中科院分区：
文献类型：
--
作者：
Inamochi, Yuko;Mochizuki, Kazuki;Goda, Toshinao
组蛋白H3在赖氨酸4（K4）处的甲基化与常染色质区域相关，并且被认为对于分化期间基因的转录激活是重要的。在这项研究中，我们发现组蛋白H3在K4的二甲基化和三甲基化，以及组蛋白H3和H4从启动子/增强子到溶质载体家族2成员5（SLC 2A 5）基因转录起始位点附近的转录区域的乙酰化，及其表达，被诱导分化的类尼古丁Caco-2细胞。这些作用伴随着这些细胞的细胞生长的接触抑制。此外，这些修改是通过与合成的糖皮质激素地塞米松和p44/42丝裂原活化蛋白激酶抑制剂PD 89059共同治疗诱导的。我们的研究结果表明，组蛋白H3在K4甲基化和乙酰化的组蛋白H3和H4参与SLC 2A 5基因诱导与肠分化的Caco-2细胞。(C)2009 Elsevier Inc. All rights reserved.
Histone H3 methylation at lysine 4 (K4) is associated with euchromatic regions and is thought to be important for the transcriptional activation of genes during differentiation. In this study, we found that di- and tri-methylation of histone H3 at K4 and acetylation of histones H3 and H4 from the promoter/enhancer to the transcribed region close to the transcription initiation site of the solute carrier family 2, member 5 (SLC2A5) gene, and its expression, were induced by differentiation of intestine-like Caco-2 cells. These effects were accompanied by contact inhibition of cell growth of these cells. Furthermore, these modifications were induced by co-treatment with a synthetic glucocorticoid hormone dexamethasone and a p44/42 mitogen-activated protein kinase inhibitor PD89059. Our results suggest that methylation of histone H3 at K4 and acetylation of histones H3 and H4 are involved in SLC2A5 gene induction associated with intestinal differentiation of Caco-2 cells. (C) 2009 Elsevier Inc. All rights reserved.