INTRACELLULAR DISTRIBUTION OF COPPER IN LIVER OF RAT
INTRACELLULAR DISTRIBUTION OF COPPER IN LIVER OF RAT
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DOI：
10.1139/o67-217
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发表时间：
1967-01-01
期刊：
影响因子：
--
通讯作者：
SOURKES, TL
中科院分区：
文献类型：
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作者：
GREGORIA.G;SOURKES, TL
大鼠全肝及其亚细胞组分中铜的绝对量随年龄的增长而降低。新生大鼠肝脏中含有大部分铜的线粒体组分和核组分的下降幅度远远大于可溶性组分和微粒体组分的下降幅度。这导致了铜的重新分配，有利于上清液。在成年大鼠中，这一部分大约是肝脏总铜含量的1/2，其次是细胞核、线粒体和微粒体的含量。腹腔注射CuSO4或饲喂低铜饲料使肝脏的铜含量分别高于和低于正常水平，并对细胞内分布产生如下影响。在铜负荷中，线粒体和细胞核占据了大部分多余的铜，细胞质和微粒体积累的很少。铜缺乏时，可溶性铜和线粒体铜的损失较大，微粒体和细胞核的铜损失较少。肝脏亚细胞部分的铜浓度似乎与器官的总铜含量有关，而与动物的生理状态无关。
The absolute amount of Cu in whole liver and its subcellular fractions decreases as the rat matures. The decrease in mitochond-rial and nuclear fractions, which contain most of the Cu in the liver of the newborn rat, is much greater than the decrease in the soluble and microsomal fractions. This results in a redistribution of the Cu, in favor of the supernatant. In the adult rat, this fraction is about 1/2 the total Cu content of the liver, with the content of nuclei, mitochondria, and microsomes following in that order. The intraperitoneal injection of CUSO4 or the feeding of a diet low in Cu brings the Cu content of the liver above and below normal levels, respectively, and affects the intracellular distribution as follows. In Cu loading, mitochondria and nuclei hold most of the excess and the cytoplasm and microsomes accumulate much less. In Cu deficiency, there is a greater loss of soluble and mitochondrial Cu and there are minor losses in microsomes and nuclei. The Cu concentration in subcellular fractions of the liver seems to be related to the total Cu content of the organ rather than to the physiological state of the animal.