IL-27 mediates immune response of pneumococcal vaccine SPY1 through Th17 and memory CD4(+)T cells.
IL-27 mediates immune response of pneumococcal vaccine SPY1 through Th17 and memory CD4(+)T cells.
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DOI：
10.1016/j.isci.2023.107464
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发表时间：
2023-08-18
期刊：
影响因子：
5.8
通讯作者：
Xu, Wenchun
中科院分区：
文献类型：
--
作者：
Zhang, Yanyu;Gao, Song;Yao, Shifei;Weng, Danlin;Wang, Yan;Huang, Qi;Zhang, Xuemei;Wang, Hong;Xu, Wenchun
疫苗接种是预防肺炎球菌疾病的有效手段，SPY1是我们之前获得的肺炎球菌减毒活疫苗。我们发现在接种 SPY1 的小鼠中 IL-27 及其特异性受体 (WSX-1) 增加。接种 SPY1 的 IL-27Rα−/− 小鼠的细菌清除率和存活率降低。 IL-27Rα−/− 小鼠中疫苗诱导的 Th17 细胞反应和 IgA 分泌也受到抑制。在用灭活的 SPY1 刺激的 IL-27Rα−/− 骨髓源性树突状细胞 (BMDC) 中，STAT3 和 NF-κB 信号传导以及 Th17 细胞极化相关细胞因子的表达也有所减少。在接种 SPY1 的 IL-27Rα−/− 小鼠中，记忆 CD4+T 细胞的数量也有所减少。这些结果表明，IL-27 通过 STAT3 和 NF-κB 信号通路促进 Th17 极化以及 SPY1 疫苗中记忆 CD4+T 细胞的产生，从而在 SPY1 疫苗中发挥保护作用。此外，我们发现SPY1疫苗的免疫保护作用与有氧糖酵解无关。 IL-27 在肺炎球菌疫苗 SPY1 中发挥保护作用 IL-27 在 SPY1 疫苗接种中促进 Th17 细胞免疫应答 IL-27 在 SPY1 疫苗接种中促进记忆 CD4+T 细胞的产生 SPY1 疫苗的免疫保护独立于糖酵解 分子生物学；免疫学；免疫反应；病毒学
Vaccination is an effective means of preventing pneumococcal disease and SPY1 is a live attenuated pneumococcal vaccine we obtained earlier. We found IL-27 and its specific receptor (WSX-1) were increased in SPY1 vaccinated mice. Bacterial clearance and survival rates were decreased in SPY1 vaccinated IL-27Rα−/− mice. The vaccine-induced Th17 cell response and IgA secretion were also suppressed in IL-27Rα−/− mice. STAT3 and NF-κB signaling and expression of the Th17 cell polarization-related cytokines were also decreased in IL-27Rα−/− bone-marrow-derived dendritic cells(BMDC) stimulated with inactivated SPY1. The numbers of memory CD4+T cells were also decreased in SPY1 vaccinated IL-27Rα−/− mice. These results suggested that IL-27 plays a protective role in SPY1 vaccine by promoting Th17 polarization through STAT3 and NF-κB signaling pathways and memory CD4+T cells production in the SPY1 vaccine. In addition, we found that the immune protection of SPY1 vaccine was independent of aerobic glycolysis. IL-27 plays a protective role in pneumococcal vaccine SPY1 IL-27 promotes Th17 cell immune response in SPY1 vaccination IL-27 promotes the production of memory CD4+T cells in SPY1 vaccination The immune protection of SPY1 vaccine is independent of glycolysis Molecular biology; Immunology; Immune response; Virology
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影响因子：
30.5
作者：
Acosta-Rodriguez, Eva V.;Napolitani, Giorgio;Sallusto, Federica
通讯作者：
Sallusto, Federica
影响因子：
4.6
作者：
Fabbi M;Carbotti G;Ferrini S
通讯作者：
Ferrini S
影响因子：
30.5
作者：
Intlekofer, AM;Takemoto, N;Reiner, SL
通讯作者：
Reiner, SL
影响因子：
8
作者：
Chen Y;Shao C;Fan NW;Nakao T;Amouzegar A;Chauhan SK;Dana R
通讯作者：
Dana R
DOI：
10.1084/jem.20091279
发表时间：
2010-01-18
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Budhu S;Loike JD;Pandolfi A;Han S;Catalano G;Constantinescu A;Clynes R;Silverstein SC
通讯作者：
Silverstein SC