Effect of "stress" on serum prolactin and luteinizing hormone levels during the estrous cycle of the rat.
Effect of "stress" on serum prolactin and luteinizing hormone levels during the estrous cycle of the rat.
复制标题
“应激”对大鼠动情周期血清催乳素和黄体生成素水平的影响。
作者：
J. D. Neill
测定了采血方法对发情周期大鼠血清催乳素和黄体生成素水平的影响。在乙醚麻醉下行腹主动脉切开术后，分别于发情周期的以下时间段处死动物5只，取腹主动脉血5只：动情间期1、2、3天下午3点，动情前期和动情前期上午10点，动情前期和动情期下午3点。与断头相比，乙醚麻醉下剖腹出血可使血清催乳素水平在整个发情周期内升高3~8倍，但在发情前期的下午，已升高的水平未见进一步升高。乙醚-开腹手术也提高了卵巢切除女性的催乳素滴度，但对男性没有。乙醚麻醉后断头动物发情当天早晨催乳素水平高于断头动物，但低于乙醚剖腹动物。血清促黄体生成素浓度不受任何一种方法的影响。
The effect of method of blood col-lection on serum prolactin and LH levels was determined throughout the estrous cycle of the rat. Five animals were decapitated and 5 were bled from the abdominal aorta following lap-arotomy under ether anesthesia at each of the following times during the estrous cycle: 3 PM on diestrus days 1, 2 and 3 and 10 AM and 3 PM on the days of proestrus and estrus. Compared to decapitation, laparotomy and bleeding under ether anesthesia induced 3- to 8-fold increases in serum prolactin values at all times studied dur-ing the estrous cycle except on the afternoon of proestrus, when no further increase in already elevated levels was observed. Ether-laparotomy also elevated prolactin titers in ovariectomized females but not in males. Prolactin levels on the morning of proestrus in animals anesthetized with ether and then decapitated were higher than in decapitated animals but less than in ether-laparotomized animals. Serum concentrations of LH were not affected by any of the procedur...