Development of an integrated automation system with a magnetic bead-mediated nucleic acid purification device for genetic analysis and gene manipulation
Development of an integrated automation system with a magnetic bead-mediated nucleic acid purification device for genetic analysis and gene manipulation
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DOI：
10.1002/bit.20049
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发表时间：
2004-06-20
影响因子：
3.8
通讯作者：
Yohda, M
中科院分区：
文献类型：
--
作者：
Akutsu, J;Tojo, Y;Yohda, M
我们已经开发了一个遗传分析和基因操作的集成自动化系统。该系统SX-8 G Plus配备有8喷嘴点样装置、热循环仪、冷却试剂储存器、4个吸头储存架、4个微孔板平台、缓冲液储存器、琼脂糖凝胶电泳装置、电源、用于更换电泳缓冲液的泵和CCD摄像机。核酸提取和纯化的自动化是自动化遗传分析和基因操作中最困难的步骤，它是使用带有Magtration技术的磁珠实现的，我们之前开发了该技术用于自动化处理顺磁性珠子。使用该系统，我们可以从样品加载开始通过琼脂糖凝胶电泳进行DNA片段的自动化分离和纯化。该系统将使遗传分析和基因操作中的几乎所有程序实现自动化。(C)2004 Wiley Periodicals，Inc.
We have developed an integrated automation system for genetic analysis and gene manipulation. The system, SX-8G Plus, is equipped with an 8-nozzle dispensing unit, a thermal cycler, a cooled reagent reservoir, four tip storage racks, four microplate platforms, buffer reservoirs, an agarose gel electrophoresis unit, a power supply, a pump for exchanging electrophoresis buffer, and a CCD camera. Automation of nucleic acid extraction and purification, the most difficult step in automating genetic analysis and gene manipulation, was realized using magnetic beads with Magtration Technology, which we have previously developed for automating the handling of paramagnetic beads. Using this system, we could perform the automated separation and purification of DNA fragments by agarose gel electrophoresis starting from sample loading. The system would enable the automation of almost all procedures in genetic analysis and gene manipulation. (C) 2004 Wiley Periodicals, Inc.