Natural Nanocolloids Mediate the Phytotoxicity of Graphene Oxide
Natural Nanocolloids Mediate the Phytotoxicity of Graphene Oxide
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天然纳米胶体介导氧化石墨烯的植物毒性
DOI：
10.1021/acs.est.9b07460
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发表时间：
2020
影响因子：
11.4
通讯作者：
Hu Xiangang
中科院分区：
文献类型：
--
作者：
Ouyang Shaohu;Zhou Qixing;Zeng Hui;Wang Yue;Hu Xiangang
纳米胶体（Ncs）在天然地表水中普遍存在。然而，Ncs对氧化石墨烯（一种流行的工程纳米材料（ENM））的命运和生态毒性的影响在很大程度上仍然未知。Ncs对氧化石墨烯具有较强的吸附亲和力（KL= 1.93 L/mg）和较高的吸附容量（176.2 mg/g）。Ncs杂交后，氧化石墨烯纳米片呈卷轴状，氧化石墨烯的聚集速率降低。比较了腐植酸和Ncs对氧化石墨烯毒性的影响。腐植酸可减轻氧化石墨烯的植物毒性。而GO和GO- ncs对藻细胞有包膜作用，均能进入藻细胞。与氧化石墨烯相比，氧化石墨烯ncs可诱导更高的活性氧（ROS）生成，更强的DNA损伤和质解，以及更明显的光合作用抑制。蛋白质组学分析显示，光系统I和ii相关蛋白（如E1ZQR2和E1ZPG5）在GO- ncs组中比GO组受到更显著的调节。蛋白质组学和代谢组学分析表明，抑制碳水化合物、脂肪酸和氨基酸代谢有助于ROS的产生。鉴于纳米粒子的高浓度和活性，上述结果强调需要重新考虑纳米粒子介导的环境行为以及纳米粒子和其他污染物的风险。
Nanocolloids (Ncs) are ubiquitous in natural surface waters. However, the effects of Ncs on the fate and ecotoxicity of graphene oxide (GO, a popular engineered nanomaterial (ENM)) remain largely unknown. Ncs exhibit strong adsorption affinity (KL= 1.93 L/mg) and high adsorption capacity (176.2 mg/g) for GO. After Ncs hybridization, GO nanosheets became scrolls, and the aggregation rate of GO decreased. The influence of humic acid and Ncs on GO toxicity was compared. Humic acid mitigated the phytotoxicity of GO. However, GO and GO-Ncs were found to have an envelopment effect on algal cells, and both could enter algal cells. GO-Ncs induced higher reactive oxygen species (ROS) generation, stronger DNA damage and plasmolysis, and more obvious inhibition of photosynthesis compared to GO. Proteomic analysis revealed that photosystem I- and II-related proteins (e.g., E1ZQR2 and E1ZPG5) were regulated more significantly in the GO-Ncs groups than in the GO groups. A combined proteomic and metabolomic analysis showed that inhibition of carbohydrate, fatty acid, and amino acid metabolism contributed to ROS generation. Given the high concentrations and activity of Ncs, the above results highlight the need for reconsideration of the Ncs-mediated environmental behaviors and risks of ENMs and other pollutants.