Identification of a novel mitogen-activated protein kinase kinase gene (MKK2) in the oilseed rape Brassica campestris
Identification of a novel mitogen-activated protein kinase kinase gene (MKK2) in the oilseed rape Brassica campestris
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DOI：
10.2478/s11756-014-0455-8
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发表时间：
2014-12
期刊：
影响因子：
1.5
通讯作者：
Tengguo Zhang;Y. Wang;Juan Wang;X. Xia;N. Yang;Yingli Yang;Wan Sun
中科院分区：
文献类型：
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作者：
Tengguo Zhang;Y. Wang;Juan Wang;X. Xia;N. Yang;Yingli Yang;Wan Sun
丝裂原活化蛋白激酶(MAPK)级联信号通路参与植物生长发育、生物和非生物胁迫反应等多种过程。丝裂原活化蛋白激酶(MAPK)是连接MPK和MPKK的通路，通过介导多种植物逆境反应，参与MAPK级联反应。然而，只有少数来自油菜的MKK被鉴定为功能蛋白。本研究从油菜中分离并鉴定了一个新基因MKK2。生物信息学分析表明，MKK2的全长为1,344个碱基，开放阅读框为1,068个碱基，编码355个氨基酸的多肽。MKK2的预测相对分子质量为39.3 kDa，等电点为6.8。实时定量聚合酶链式反应分析表明，MKK2在寒冷和盐条件下均可诱导表达。Western印迹分析表明，冷、盐和UV-B辐射均可诱导MKK2蛋白的表达。MKK2蛋白定位于细胞核。这些结果表明，MKK2在植物对低温和盐胁迫的反应中起着重要的调节作用。
Mitogen-activated protein kinase (MAPK) cascades participate in various processes, including plant growth and development as well as biotic and abiotic stress responses. MAPK kinases (MKKs), which link MPKs and MPKK kinases, are involved in MAPK cascades by mediating various plant stress responses. However, only a few MKKs from Brassica campestris (rape) have been functionally characterized. This study delivers the results from isolation and characterization of a novel gene, MKK2, from rape. Bioinformatics analysis revealed that the cDNA length of MKK2 is 1,344 bp with an open reading frame of 1,068 bp, which encodes a polypeptide containing 355 amino acids. The obtained MKK2 exhibited a predicted molecular mass of 39.3 kDa and an isoelectric point of 6.8. Quantitative real-time polymerase chain reaction analysis revealed that MKK2 expression can be induced by cold and salt. Western blot analysis revealed that MKK2 protein expression can be induced by cold, salt, and UV-B radiation. The MKK2 protein was localized in the nucleus. These results suggest that MKK2 is important for the regulation of cold- and salt-stress responses in plants.