Formulation of enamel matrix derivative for surface coating - Kinetics and cell colonization
Formulation of enamel matrix derivative for surface coating - Kinetics and cell colonization
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DOI：
10.1111/j.1600-051x.1997.tb00249.x
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发表时间：
1997-09-01
影响因子：
6.7
通讯作者：
Hammarstrom, L
中科院分区：
文献类型：
--
作者：
Gestrelius, S;Andersson, C;Hammarstrom, L
釉质基质衍生物（EMD）含有属于釉原蛋白家族的蛋白质复合物。牙釉质基质以及EMD已被发现，促进牙周再生时，适用于裸露的根面开裂模型。在目前的研究中，它表明，藻酸丙二醇酯（PGA）是一个合适的车辆EMD的局部应用。在酸性pH值下，EMD可以溶解在PGA中，产生高粘性溶液。在中性pH和体温下，粘度降低，EMD沉淀。已使用椭圆偏振法、全内反射荧光（TIRF）和生物特异性相互作用分析（BIA）分析了EMD在矿物或蛋白质表面的多层膜。研究表明EMD对羟基磷灰石和胶原蛋白以及裸露的牙根都有吸附作用。它形成不溶性的球形复合物，并且可检测的量在牙根表面的施用部位保留两周，如大鼠和猪的放射性标记蛋白质所示。对猴牙齿的扫描电子显微镜（SEM）研究进一步表明，PGA中的EMD可能在应用后的第一周内促进成纤维细胞样细胞的再增殖。
Enamel Matrix Derivative (EMD) contains a protein complex belonging to the amelogenin family. Enamel matrix as well as EMD have been found to promote periodontal regeneration when applied onto denuded root surfaces in dehiscence models. In the present studies it is shown that propylene glycol alginate (PGA) is a suitable vehicle for EMD for its local application. EMD can be dissolved in PGA at an acidic pH, resulting in a highly viscous solution. At neutral pH and body temperature the viscosity decreases and EMD precipitates, Multilayers of EMD on mineral or protein surfaces have been analysed using ellipsometry, total internal reflection fluorescence (TIRF) and biospecific interaction analysis (BIA). The studies show that EMD adsorbs both to hydroxyapatite and collagen and to denuded dental roots. It forms insoluble spherical complexes, and detectable amounts remain at the site of application on the root surface for two weeks, as shown with radiolabelled protein in rats and pigs. Scanning electron micrograph (SEM) studies on monkey teeth further indicate that EMD in PGA may promote repopulation of fibroblast-like cells during the first weeks after application.