Genome-Wide Identification of bZIP Family Genes Involved in Drought and Heat Stresses in Strawberry (Fragaria vesca).
Genome-Wide Identification of bZIP Family Genes Involved in Drought and Heat Stresses in Strawberry (Fragaria vesca).
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DOI：
10.1155/2017/3981031
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发表时间：
2017
影响因子：
2.9
通讯作者：
Cheng ZM
中科院分区：
文献类型：
--
作者：
Wang XL;Chen X;Yang TB;Cheng Q;Cheng ZM
碱性亮氨酸拉链（bZIP）基因在植物的各种生理过程以及生物或非生物胁迫中起着重要的作用。根据草莓bZIP蛋白与拟南芥和水稻的系统发育关系，将其分为10个进化枝，并对50个bZIP基因进行了鉴定和分析。在bZIP结构域的基本和铰链区内观察到五类内含子模式。另外还发现了一些具有组特异性的保守基序。此外，我们预测的DNA结合特异性的基本和铰链区，以及亮氨酸拉链区的二聚化特性，这是与我们的系统进化支一致，并分为20个亚科。在15个器官和两种类型的果实的不同发育阶段，进化枝A bZIP成员表现出不同的组织特异性表达模式和重复的基因的差异调节，表明功能的多样化，再加上该基因家族在草莓中的扩展。在正常生长条件下，进化枝A的mrna11837和mrna30280在器官和果实中的表达量很低，但在干旱和高温处理后，不同基因组的表达量均有所提高，这可能是由于干旱和高温处理之间的反应途径不同所致。
Basic leucine zipper (bZIP) genes are known to play a crucial role in response to various processes in plant as well as abiotic or biotic stress challenges. We have performed an identification and characterization of 50 bZIP genes across the woodland strawberry (Fragaria vesca) genome, which were divided into 10 clades according to the phylogenetic relationship of the strawberry bZIP proteins with those in Arabidopsis and rice. Five categories of intron patterns were observed within basic and hinge regions of the bZIP domains. Some additional conserved motifs have been found with the group specificity. Further, we predicted DNA-binding specificity of the basic and hinge regions as well as dimerization properties of leucine zipper regions, which was consistent with our phylogenetic clade and classified into 20 subfamilies. Across the different developmental stages of 15 organs and two types of fruits, the clade A bZIP members showed different tissue-specific expression patterns and the duplicated genes were differentially regulated, indicating a functional diversification coupled with the expansion of this gene family in strawberry. Under normal growth conditions, mrna11837 and mrna30280 of clade A showed very weak expression levels in organs and fruits, respectively; but higher expression was observed with different set of genes following drought and heat treatment, which may be caused by the separate response pathway between drought and heat treatments.