Mutations of the Apc gene in experimental colorectal carcinogenesis induced by azoxymethane in F344 rats
Mutations of the Apc gene in experimental colorectal carcinogenesis induced by azoxymethane in F344 rats
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DOI：
10.1038/bjc.1998.359
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发表时间：
1998-06-01
影响因子：
8.8
通讯作者：
Dolara, P
中科院分区：
文献类型：
--
作者：
De Filippo, C;Caderni, G;Dolara, P
我们在大鼠中研究了Ape基因的作用，Ape基因在家族性腺瘤性息肉病和散发性结肠癌中发生突变，导致从正常结肠粘膜到异常隐窝灶（ACF），最后到腺瘤和腺癌的过程。我们使用体外合成蛋白质测定法分析了66例ACF和28例氧化偶氮甲烷诱导的结肠谣言中大鼠Ape基因第15号外显子的突变。ACF中没有发现Ape突变，而谣言中发现了5个突变，数据表明Ape基因的突变与ACF向腺瘤和腺癌的转变有关，而与从正常粘膜向ACF的转变无关。
We investigated in the rat the role of the Ape gene, which is mutated in familial adenomatous polyposis and sporadic colon cancer in the process leading from normal colonic mucosa to aberrant crypt foci (ACF) and finally to adenomas and adenocarcinomas. We analysed mutations in exon 15 of the rat Ape gene using in vitro synthesized protein assay in 66 ACF and in 28 colon rumours induced by azoxymethane. No Ape mutations were found in ACF, whereas five mutations were found in the rumours, The data suggest that mutations of the Ape gene are associated with the transition from ACF to adenoma and adenocarcinoma and not from normal mucosa to ACF.