Early events of SARS coronavirus infection in Vero cells
Early events of SARS coronavirus infection in Vero cells
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DOI：
10.1002/jmv.10499
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发表时间：
2003-11-01
影响因子：
12.7
通讯作者：
Ling, AE
中科院分区：
文献类型：
--
作者：
Ng, ML;Tan, SH;Ling, AE
最近在新加坡爆发的严重急性呼吸系统综合症 (SARS) 中，使用从一名患者身上分离出来的病毒来感染 Vero E6 细胞。这项研究集中在感染的前 30 分钟。研究发现，SARS 冠状病毒在 30 分钟内附着、进入并脱去核衣壳。感染后 5 分钟，Vero 细胞质膜上排列着一些病毒颗粒。病毒颗粒在细胞表面处于不同的融合阶段，因为进入细胞不是一个同步过程。进入后（10 和 15 分钟），球形核心颗粒移动到大液泡内的细胞质中。令人惊讶的是，在感染的如此早期阶段（20 分钟），病毒诱导的受感染细胞的变化是显而易见的。在与核心颗粒相同的液泡内，髓磷脂样膜轮的诱导是明显的。目前尚不清楚这种病毒引起的变化的意义。感染后 25-30 分钟 (p.i.)，球形核心颗粒似乎已解离，取而代之的是甜甜圈形电子致密结构。这些可能是病毒基因组和螺旋核衣壳。它们不再处于大液泡中，而是包装成细胞质中较小的液泡，有时也成小群。
An isolate from a patient in the recent severe acute respiratory syndrome (SARS) outbreak in Singapore was used to infect Vero E6 cells. This study concentrated on the first 30 min of infection. It was discovered that the SARS coronavirus attached, entered, and uncoated the nucleocapsids, all within a 30-min period. At 5 min after infection, several virus particles lined the Vero cell plasma membrane. Virus particles were at various stages of fusion at the cell surface, since entry was not a synchronised process. After entry (10 and 15 min), spherical core particles moved into the cytoplasm within large vacuoles. Quite surprising at such early stages of infection (20 min), a virus-induced change in the infected cells was evident. The induction of myelin-like membrane whorls was obvious within the same vacuoles as the core particles. The significance of this virus-induced change is unknown at this stage. By 25-30 min postinfection (p.i.), the spherical core particles appeared to be disassociating and, in their place, doughnut-shaped electron-dense structures were observed. These could be the virus genomes together with the helical nucleocapsids. They were no longer in large vacuoles but packaged into smaller vacuoles in the cytoplasm, and occasionally in small groups.