Proofreading and aminoacylation of tRNAs before export from the nucleus
Proofreading and aminoacylation of tRNAs before export from the nucleus
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DOI：
10.1126/science.282.5396.2082
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发表时间：
1998-12-11
期刊：
影响因子：
56.9
通讯作者：
Dahlberg, JE
中科院分区：
文献类型：
--
作者：
Lund, E;Dahlberg, JE
在细胞核中合成和加工后，成熟的转运RNA（tRNA）以三磷酸鸟苷依赖的方式输出到细胞质。通过监测细胞核中tRNA的结构和功能来避免缺陷或不成熟tRNA的输出，并且仅输出具有成熟5'和3'末端的tRNA。所有的tRNA检查可以在非洲爪蟾卵母细胞的细胞核中被氨酰化，从而提供了一个可能的机制，新制作的tRNA的功能校对。抑制特定tRNA的氨酰化会延迟其在细胞质中的出现，表明核氨酰化促进有效输出。
After synthesis and processing in the nucleus, mature transfer RNAs (tRNAs) are exported to the cytoplasm in a Ran.guanosine triphosphate-dependent manner. Export of defective or immature tRNAs is avoided by monitoring both structure and function of tRNAs in the nucleus, and only tRNAs with mature 5' and 3' ends are exported. ALL tRNAs examined can be aminoacylated in nuclei of Xenopus oocytes, thereby providing a possible mechanism for functional proofreading of newly made tRNAs. Inhibition of aminoacylation of a specific tRNA retards its appearance in the cytoplasm, indicating that nuclear aminoacylation promotes efficient export.