Molecular cloning and gene expression of Foxl2 in the Nile tilapia, Oreochromis niloticus.
Molecular cloning and gene expression of Foxl2 in the Nile tilapia, Oreochromis niloticus.
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DOI：
10.1016/j.bbrc.2004.05.133
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发表时间：
2004-07
影响因子：
3.1
通讯作者：
Deshou Wang;Tohru Kobayashi;Linyan Zhou;Y. Nagahama
中科院分区：
文献类型：
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作者：
Deshou Wang;Tohru Kobayashi;Linyan Zhou;Y. Nagahama
通过RT-PCR和RACE技术从尼罗罗非鱼卵巢中克隆到Fox 12基因。来自脊椎动物的已知Foxl 2序列的比对证实了Foxl 2开放阅读框和蛋白质序列的保守性，特别是叉头结构域和C-末端区域，而一些氨基酸的均聚物序列仅在哺乳动物中发现，而在非哺乳动物脊椎动物中未发现。RT-PCR结果显示，Foxl 2在罗非鱼脑（B）、垂体（P）、鳃和性腺（G）中均有表达，卵巢中表达量最高，反映Foxl 2参与B-P-G轴的调控。北方杂交和原位杂交也显示了明显的性二态表达模式。Foxl 2 mRNA主要在卵母细胞周围的颗粒细胞中检测到。Foxl 2在罗非鱼卵巢中的表达在性腺分化的早期开始，并持续到成年，这意味着Foxl 2参与鱼类性腺分化和卵巢功能的维持。
A Foxl2 cDNA was cloned from the Nile tilapia ovary by RT-PCR and subsequent RACE. Alignment of known Foxl2 sequences from vertebrates confirmed the conservation of the Foxl2 open reading frame and protein sequences, especially the forkhead domain and C-terminal region, while some homopolymeric runs of amino acids are found only in mammals but not in non-mammalian vertebrates. RT-PCR revealed that Foxl2 is expressed in the tilapia brain (B), pituitary (P), gill, and gonads (G), with the highest level of expression in the ovary, reflecting the involvement of Foxl2 in B–P–G axis. Northern blotting and in situ hybridization also revealed an evident sexual dimorphic expression pattern in the gonads. Foxl2 mRNA was mainly detected in the granulosa cells surrounding the oocytes. The ovarian expression of Foxl2 in tilapia begins early during the differentiation of the gonads and persists until adulthood, implying the involvement of Foxl2 in fish gonad differentiation and the maintenance of ovarian function.