Effect of temperature and relative humidity on the stability of infectious porcine reproductive and respiratory syndrome virus in aerosols
Effect of temperature and relative humidity on the stability of infectious porcine reproductive and respiratory syndrome virus in aerosols
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DOI：
10.1051/vetres:2006044
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发表时间：
2007-01-01
影响因子：
4.4
通讯作者：
Zimmerman, Jeffrey
中科院分区：
文献类型：
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作者：
Hermann, Joseph;Hoff, Steve;Zimmerman, Jeffrey
本实验的目的是描述空气传播的猪繁殖与呼吸综合征病毒（PRRSV）的稳定性作为温度和相对湿度的函数。使用24喷嘴Collison雾化器将感染性PRRSV的云雾化到维持在特定温度和相对湿度下的动态气溶胶环形（DAT）中。使用SKC BioSampler（（R））撞击器随时间重复采样DAT内的PRRSV云，并测定空气样品中的总病毒RNA（RT-PCR）和传染性PRRSV浓度（TCID 50）。如通过定量RT-PCR所测量的，PRRSV RNA在本研究中评价的条件下是稳定的。因此，比较病毒RNA和罗丹明B染料（一种物理示踪剂），发现线的斜率没有显著差异。将感染性病毒的滴度按时间作图，并使用线性回归分析计算感染性PRRSV的半衰期（T1/2）。结果表明，气溶胶化的PRRSV在较低的温度和/或相对湿度下更稳定，但温度对T1/2的影响大于相对湿度。基于这些结果，推导出一个方程来预测的T1/2的传染性气传PRRSV的任何组合的环境温度和相对湿度。
The objective of this experiment was to describe the stability of airborne infectious porcine reproductive and respiratory syndrome virus ( PRRSV) as a function of temperature and relative humidity. A cloud of infectious PRRSV was aerosolized using 24-jet Collison nebulizer into a dynamic aerosol toroid (DAT) maintained at a specific temperature and relative humidity. The PRRSV cloud within the DAT was sampled repeatedly over time using SKC BioSampler((R)) impingers and the total viral RNA (RT-PCR) and concentration of infectious PRRSV (TCID50) in the air samples was determined. As measured by quantitative RT-PCR, PRRSV RNA was stable under the conditions evaluated in this study. Thus, a comparison of viral RNA and Rhodamine B dye, a physical tracer, found no significant difference in the slopes of the lines. Titers of infectious virus were plotted by time and the half-life (T1/2) of infectious PRRSV was calculated using linear regression analysis. An analysis of the results showed that aerosolized PRRSV was more stable at lower temperatures and/or lower relative humidity, but temperature had a greater effect on the T1/2 of PRRSV than relative humidity. Based on these results, an equation was derived to predict the T1/2 of infectious airborne PRRSV for any combination of environmental temperature and relative humidity.