Sulfolobus tokodaii ST0053 Produces a Novel Thermostable, NAD-Dependent Medium-Chain Alcohol Dehydrogenase
Sulfolobus tokodaii ST0053 Produces a Novel Thermostable, NAD-Dependent Medium-Chain Alcohol Dehydrogenase
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DOI：
10.1128/aem.01392-08
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发表时间：
2009-01
影响因子：
4.4
通讯作者：
H. Yanai;Katsumi Doi;T. Ohshima
中科院分区：
文献类型：
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作者：
H. Yanai;Katsumi Doi;T. Ohshima
摘要在大肠杆菌中表达了东京硫化叶菌乙醇脱氢酶（ADH）基因ST 0053。纯化的重组酶是一种依赖于NAD的中链ADH，具有高的热稳定性和宽范围的pH耐受性。这是在S. tokodaii基因组。
ABSTRACT An alcohol dehydrogenase (ADH) gene, ST0053, from Sulfolobus tokodaii was expressed in Escherichia coli. The purified recombinant enzyme was an NAD-dependent medium-chain ADH with high thermostability and tolerance of a wide range of pHs. This is the first step in creating an experimental functionality library of 10 genes annotated as ADHs in the S. tokodaii genome.