Enhanced Chloride Cell Turnover in the Gills of Chum Salmon Fry in Seawater
Enhanced Chloride Cell Turnover in the Gills of Chum Salmon Fry in Seawater
复制标题
海水中鲑鱼鱼苗鳃中氯细胞更新率的提高
DOI：
10.2108/zsj.13.655
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
T. Kaneko
中科院分区：
文献类型：
--
作者：
K. Uchida;T. Kaneko
摘要以5-溴-2 ′-脱氧尿苷（BrdU）为新分化细胞的标记，研究了淡水和海水适应的大麻哈鱼（Oncorhynchus凯塔）鱼苗和海水适应的鱼苗鳃氯细胞的分化和更新。氯细胞和BrdU标记的细胞核分别用Na+，K+-ATP酶和BrdU特异性抗血清进行免疫细胞化学检测。虽然位于板层和板层间区域（细丝氯细胞）的基础上的氯细胞的数量是恒定的FW，SW和SW转移组，位于板层上皮（板层氯细胞）的氯细胞的数量在SW比FW少，并在SW适应过程中减少。BrdU阳性细胞主要分布在纤维中，在板层中很少见到。在前24小时内，FW，SW和SW转移鱼的丝状氯化物细胞的周转率分别为8%，21%和28%。这些结果表明，氯细胞在细丝中不断更换新分化的细胞在FW和SW，和营业额约3倍以上，在SW比FW。更频繁的营业额丝状氯化物细胞在SW表明一个特定的作用，大概是在盐排泄。
Abstract Cellular differentiation and renewal in the gill chloride cells were examined in freshwater (FW) and seawater (SW)-adapted chum salmon (Oncorhynchus keta) fry and in fry during SW adaptation using 5-bromo-2′-deoxyuridine (BrdU) as a marker for newly-differentiated cells. Chloride cells and BrdU-labeled nuclei were immunocytochemically detected by using antisera specific for Na+,K+-ATPase and BrdU, respectively. Although the number of chloride cells located at the base of the lamellae and in the interlamellar region (filament chloride cells) was constant in FW, SW and SW-transferred groups, chloride cells located in the lamellar epithelium (lamellar chloride cells) were fewer in SW than in FW, and decreased during SW adaptation. Newly-differentiated cells with BrdU-immunoreactive nuclei were detected mainly in the filaments, and rarely observed in the lamellae. The turnover rates of filament chloride cells for FW, SW and SW-transferred fish during the first 24 hr were 8%, 21% and 28%, respectively. These results indicate that chloride cells in the filament are replaced continuously by newly-differentiated cells in both FW and SW, and that the turnover was about 3 times greater in SW than in FW. More frequent turnover of filament chloride cells in SW suggests a specific role, presumably in salt excretion.
影响因子：
56.9
作者：
FOSKETT, JK;SCHEFFEY, C
通讯作者：
SCHEFFEY, C