Self-organization of Nucleic Acids in Lipid Constructs.
Self-organization of Nucleic Acids in Lipid Constructs.
复制标题
DOI：
10.1016/j.cocis.2016.09.006
复制
发表时间：
2016-12
影响因子：
8.9
通讯作者：
Leal C
中科院分区：
文献类型：
--
作者：
Kang M;Kim H;Leal C
脂质和核酸（NAs）可以在层次上自组织成各种越来越复杂的几何形状的纳米结构，如1D片层相、2D六边形相和3D双连续立方相。这些脂质na组件的多样性和复杂性从基础的角度来看是有趣的，并且与基因传递和基因沉默应用的性能有关。不仅脂质na结构的化学组成，而且其实际的超分子组织影响其基因转染和沉默效率，这一发现激励了物理学家、化学家和工程师进入这一研究领域。目前，不同的脂质na结构究竟是如何与细胞和细胞器相互作用以产生最佳反应的，仍然是一个悬而未决的问题。本文综述了我们目前对不同脂质- na复合物结构及其细胞相互作用机制的认识。本文将介绍脂基NA载体设计的最新进展，重点介绍结构-功能关系。
Lipids and nucleic acids (NAs) can hierarchically self-organize into a variety of nanostructures of increasingly complex geometries such as the 1D lamellar, 2D hexagonal, and 3D bicontinuous cubic phases. The diversity and complexity of those lipid–NA assemblies are interesting from a fundamental perspective as well as being relevant to the performance in gene delivery and gene silencing applications. The finding that not only the chemical make of the lipid-NA constructs, but their actual supramolecular organization, affects their gene transfection and silencing efficiencies has inspired physicists, chemists, and engineers to this field of research. At the moment it remains an open question how exactly the different lipid-NA structures interact with cells and organelles in order to output an optimal response. This article reviews our current understanding of the structures of different lipid–NA complexes and the corresponding cellular interaction mechanisms. The recent advances in designing optimal lipid–based NA carriers will be introduced with an emphasis on the structure–function relations.