Epidermal dysplasia and abnormal hair follicles in transgenic mice overexpressing homeobox gene MSX-2
Epidermal dysplasia and abnormal hair follicles in transgenic mice overexpressing homeobox gene MSX-2
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DOI：
10.1046/j.1523-1747.1999.00680.x
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发表时间：
1999-08-01
影响因子：
6.5
通讯作者：
Chuong, CM
中科院分区：
文献类型：
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作者：
Jiang, TX;Liu, YH;Chuong, CM
同源框基因 Msx-2 在皮肤附属器形成部位特异性表达。为了探索其在皮肤形态发生中的作用，我们培育了在巨细胞病毒启动子控制下表达 Msx-2 的转基因小鼠。这些转基因小鼠的皮肤呈片状，出现脱屑和较短的毛发。组织学分析显示表皮增厚并过度增殖，仅限于基底层。角化过度也很明显。基底层上细胞的大片区域错位并共表达角蛋白 14 和 10。基底层中整合素 β 1 和 DCC 的表达减少。毛囊与萎缩的基质区域错位。真皮显示出增加的细胞结构和空泡。我们认为 Msx-2 参与皮肤和皮肤附属器的生长控制。
The homeobox gene Msx-2 is expressed specifically in sites of skin appendage formation. To explore its part in skin morphogenesis, we produced transgenic mice expressing Msx-2 under the control of the cytomegalovirus promoter. The skin of these transgenic mice was flaky, exhibiting desquamation and shorter hairs. Histologic analysis showed thickened epidermis with hyperproliferation, which was restricted to the basal layer. Hyperkeratosis was also evident. A wide zone of suprabasal cells were misaligned and coexpressed keratins 14 and 10. There was reduced expression of integrin beta 1 and DCC in the basal layer. Hair follicles were misaligned with a shrunken matrix region. The dermis showed increased cellularity and empty vacuoles. We suggest that Msx-2 is involved in the growth control of skin and skin appendages.