Oxidative stress-associated vascular aging is xanthine oxidase-dependent but not NAD(P)H oxidase-dependent.
Oxidative stress-associated vascular aging is xanthine oxidase-dependent but not NAD(P)H oxidase-dependent.
复制标题
氧化应激相关的血管老化依赖于黄嘌呤氧化酶，但不依赖于 NAD(P)H 氧化酶。
DOI：
10.1097/01.fjc.0000245402.62864.0a
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发表时间：
2006
影响因子：
3
通讯作者：
Oyekan,Adebayo
中科院分区：
文献类型：
--
作者：
Newaz,MohammadA;Yousefipour,Zivar;Oyekan,Adebayo
血管老化的特征是内皮功能障碍，这主要是由于超氧化物产生增加，其确切来源仍然不明确。本研究比较了NAD（P）H和黄嘌呤氧化酶（XO）系统作为超氧化物来源和衰老中受损的血管功能。使用雄性Sprague道利大鼠，4月龄（年轻）和18月龄（衰老）。与年轻大鼠相比，老龄组的收缩压较高（36±3%），这伴随着乙酰胆碱诱导的肾血管舒张减少。老年大鼠尿亚硝酸盐排泄量减少（P< 0.05），这与主动脉一氧化氮合酶（NOS）活性降低及eNOS和iNOS蛋白表达减少有关。与年轻大鼠相比，老年大鼠的自由基生成增加约2倍，血浆8-异前列腺素水平增加明显，蛋白尿增加约4倍。血管NADP（H）氧化酶在两组之间没有变化，NAD（P）H氧化酶的p67 phox或p47 phox组分的表达也没有变化。而老龄大鼠主动脉XO活性增加（19±1%; P< 0.05），XO表达增加。这些结果表明，增加的自由基产生相关的老年大鼠SBP的增加是XO，而不是NAD（P）H氧化酶依赖性。
Vascular aging is characterized by endothelial dysfunction that is primarily attributed to increased superoxide production, the exact source of which remains ambiguous. This study compared the NAD (P) H and xanthine oxidase (XO) systems as sources of superoxide and impaired vascular function in aging. Male Sprague Dawley rats, 4-months-old (young) and 18-months-old (Aging), were used. Systolic blood pressure was higher (36±3%) in the aging group compared with young rats, and this was accompanied by reduced acetylcholine-induced renal vasodilatation. Urinary excretion of nitrite was lower in the aging rats (P< 0.05), and this was associated with reduced nitric oxide synthase (NOS) activity and reduced eNOS and iNOS protein expression in the aorta. Aged rats showed an approximately twofold increase in free radical generation, as evident by increased plasma 8-isoprostane level, and an approximately fourfold increase in proteinuria compared with the young rats. Vascular NADP (H) oxidase was unchanged between both groups, as was the expression of p67phox or p47phox components of NAD (P) H oxidase. However, XO activity was increased (19±1%; P< 0.05) as well as XO expression in the aorta of aging rats. These results suggest that increased free radical generation-associated increase in SBP in aging rats is XO but not NAD (P) H oxidase-dependent.