METALLOINTERCALATION REAGENTS - 2-HYDROXYETHANETHIOLATO(2,2',2''-TERPYRIDINE)-PLATINUM(II) MONOCATION BINDS STRONGLY TO DNA BY INTERCALATION
METALLOINTERCALATION REAGENTS - 2-HYDROXYETHANETHIOLATO(2,2',2''-TERPYRIDINE)-PLATINUM(II) MONOCATION BINDS STRONGLY TO DNA BY INTERCALATION
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DOI：
10.1073/pnas.71.10.3839
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
BAUER, WR
中科院分区：
文献类型：
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作者：
JENNETTE, KW;LIPPARD, SJ;BAUER, WR
红色阳离子配合物2-羟基-乙硫代乙醇（2,2 '，2″-三吡啶）铂（II）， [(terpy)-Pt(SCH2CH2OH)]+，通过插层机制与DNA紧密结合。荧光光谱研究表明，铂配合物可竞争性地抑制插入染料溴化乙啶与小牛胸腺DNA的结合。这种铂复合物增加了小牛胸腺DNA的粘度，将熔化温度提高了5°，并且在与DNA结合时表现出诱导的圆二色性。来自噬菌体PM2的封闭环状病毒DNA被复合体以类似于溴化乙锭的方式展开，其展开角度似乎比溴化乙锭略小。对相关配合物[(terpy)PtCl]+的研究表明，它具有取代性更不稳定的氯配体，也可以插入，特别是在[DNA-P]:[Pt]比大于2时。讨论了这些新型金属嵌入试剂作为重原子探针在纤维衍射或相互作用过程的电子显微镜研究中的潜在用途。
The red, cationic complex 2-hydroxy-ethanethiolato(2,2′,2″-terpyridine)platinum(II), [(terpy)-Pt(SCH2CH2OH)]+, binds strongly to DNA by a mechanism involving intercalation. By means of fluorescence spectroscopy, the platinum complex was shown to inhibit competitively the binding of the intercalating dye ethidium bromide to calf thymus DNA. This platinum complex increases the viscosity of calf thymus DNA, raises the melting temperature by up to 5°, and exhibits induced circular dichroism when bound to the DNA. The closed circular viral DNA from bacteriophage PM2 is unwound by the complex in a manner that is similar to that of ethidium bromide and with an unwinding angle that appears to be slightly less than that of ethidium. Studies of the related complex [(terpy)PtCl]+, which has a substitutionally more labile chloride ligand, suggest that it also intercalates, especially at [DNA-P]:[Pt] ratios greater than 2. The potential utility of these new metallointercalation reagents as heavy atom probes in fiber diffraction or electron microscopic studies of the interaction process is discussed.