STRESS AND ACTH INCREASE CIRCULATING CONCENTRATIONS OF 3-ALPHA-ANDROSTANEDIOL IN FEMALE RATS
STRESS AND ACTH INCREASE CIRCULATING CONCENTRATIONS OF 3-ALPHA-ANDROSTANEDIOL IN FEMALE RATS
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DOI：
10.1016/0024-3205(92)90157-k
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发表时间：
1992-01-01
期刊：
影响因子：
6.1
通讯作者：
KORNBERG, E
中科院分区：
文献类型：
--
作者：
ERSKINE, MS;KORNBERG, E
进行本实验以确定什么生理条件导致5 α-雄甾烷-3 α，17 β-二醇（3 α-雄甾烷醇，3 α-Adiol）血清水平急性升高，这在交配后15 - 30分钟内在完整发情雌性大鼠中观察到（1）。在注射外源性激素或开始应激程序之前即刻和之后20分钟从发情前期大鼠获得血液样品，并通过RIA测量这些样品中的3 α-Adiol和/或孕酮（P）的血浆浓度。静脉注射绵羊LH（5、15或45 μ g）或生理盐水导致注射后20分钟血浆3 α-Adiol显著增加。相反，地塞米松预处理大鼠静脉注射0（酸性生理盐水溶剂）、2、4或8 ng ACTH 1 - 24后，观察到3 α-Adiol呈剂量依赖性增加。最高ACTH 1 - 24剂量也导致血浆P浓度显著增加。在第三个实验中，在束缚应激开始后20分钟，观察到3 α-Adiol浓度显著高于基线;在这种情况下，血浆P浓度没有显著增加。最后，在卵巢切除术（ovx）、肾上腺切除术（adx）或ovx + adx动情间期后两天，在乙醚/颈静脉穿刺应激后采集血样。在完整的假手术和非手术对照组中，血浆3 α-Adiol对应激的反应是正常的，但在ovx和adx组中显著降低至完整动物所示的28.4%。在ovx + adx组中获得的22/26个样本中，循环3 α-Adiol浓度检测不到。这些数据表明，3 α-Adiol的血浆浓度在应激或ACTH而不是LH的反应中增加，并且卵巢和肾上腺都有助于这种增加。
The present experiments were carried out to determine what physiological conditions are responsible for the acute increases in serum levels of 5alpha-androstane-3alpha,17beta-diol (3alpha-androstanediol, 3alpha-Adiol) which are seen in the intact estrous female rat within 15-30 min after mating (1). Blood samples were obtained from proestrus rats immediately before and 20 min after injection of exogenous hormones or initiation of stress procedures, and plasma concentrations of 3alpha-Adiol and/or progesterone (P) were measured in these samples by RIA. Intravenous injections of ovine LH (5, 15, or 45 mug) or saline resulted in equivalent significant increases in plasma 3alpha-Adiol 20 min after injection. In contrast, dose-dependent increases in 3alpha-Adiol were seen after intravenous injection of 0 (acidic saline vehicle), 2, 4, or 8 ng ACTH1-24 to dexamethasone-pretreated rats. The highest ACTH1-24 dose also resulted in a significant increase in plasma P concentration. In a third experiment, a significant increase in 3alpha-Adiol concentration above baseline was seen at 20 min after onset of restraint stress; in this case plasma P concentrations did not increase significantly. Finally, blood samples were obtained after onset of ether/jugular venipuncture stress two days after ovariectomy (ovx), adrenalectomy (adx), or ovx+adx on diestrus. The plasma 3alpha-Adiol response to stress was normal in intact sham-operated and non-operated groups of controls, but was significantly diminished in the ovx and the adx groups to 28.4% of that shown by the intact animals. Circulating 3alpha-Adiol concentrations were undetectable in 22/26 samples obtained in the ovx+adx group. These data demonstrate that plasma concentrations of 3alpha-Adiol increase in response to stress or ACTH but not LH, and that both the ovary and the adrenal contribute to this increase.